Abstract
The presence of carbohydrates on proteins of Borrelia burgdorferi, the causative agent of Lyme disease, was investigated by using a digoxigenin labeling method together with Schiff staining and N-glycosidase F assay. The two major outer surface exposed proteins of 31 kDa and 34 kDa showed to be glycosylated and gel filtration high pressure liquid chromatography (HPLC) of proteins of B. burgdorferi metabolically labeled with 14C-N-acetylglucosamine revealed the incorporation of the carbohydrate into the glycosyl residue of these proteins.
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Abbreviations
- N-glycosidase F:
-
peptide-N-glycosidase F (EC 3.5.1.52)
- SDS-PAGE:
-
sodium dodecyl sulphate polyacrylamide gel electrophoresis
- WB:
-
Western blotting
- HPLC:
-
high pressure liquid chromatography
- SDS:
-
sodium dodecyl sulphate
- mAb:
-
monoclonal antibody
- MIAF:
-
mouse immune ascitic fluid
- SS:
-
Schiff staining
- Osp:
-
Outer surface protein
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Sambri, V., Stefanelli, C. & Cevenini, R. Detection of glycoproteins in Borrelia burgdorferi . Arch. Microbiol. 157, 205–208 (1992). https://doi.org/10.1007/BF00245150
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DOI: https://doi.org/10.1007/BF00245150