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Effect of toluene-permeabilization on oxidation of D-sorbitol to L-sorbose by Gluconobacter suboxydans cells immobilized in calcium alginate

Biotechnology Letters volume 16, pages 345–348 (1994)Cite this article

Summary

The effect of permeabilization of G. suboxydans cells with toluene on the oxidation of D-sorbitol to L-sorbose was investigated. Treatment of the cells with 10% toluene resulted in a three fold increase in the specific sorbitol dehydrogenase activity and a two fold increase in the efficiency of D-sorbitol conversion to L-sorbose of the free cell suspension. When the permeabilized cells were immobilized in calcium alginate, the operational stability during air-lift reactor operation was also found to increase with up to three times longer half-life(44 days) of catalytic activity compared with immobilized intact cells.

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Authors and Affiliations

  1. Applied Microbiology Research Group, Genetic Engineering Research Institute, KIST, P.O. Box 115, 305-600, Yusong, Taejon, Korea

    Young-Min Park, Eui-Sung Choi & Sang-Ki Rhee

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  1. Young-Min Park
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  2. Eui-Sung Choi
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  3. Sang-Ki Rhee
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Park, YM., Choi, ES. & Rhee, SK. Effect of toluene-permeabilization on oxidation of D-sorbitol to L-sorbose by Gluconobacter suboxydans cells immobilized in calcium alginate. Biotechnol Lett 16, 345–348 (1994). https://doi.org/10.1007/BF00245048

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  • DOI: https://doi.org/10.1007/BF00245048

Keywords

  • Toluene
  • Catalytic Activity
  • Alginate
  • Sorbitol
  • Dehydrogenase Activity