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Effect of toluene-permeabilization on oxidation of D-sorbitol to L-sorbose by Gluconobacter suboxydans cells immobilized in calcium alginate

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Summary

The effect of permeabilization of G. suboxydans cells with toluene on the oxidation of D-sorbitol to L-sorbose was investigated. Treatment of the cells with 10% toluene resulted in a three fold increase in the specific sorbitol dehydrogenase activity and a two fold increase in the efficiency of D-sorbitol conversion to L-sorbose of the free cell suspension. When the permeabilized cells were immobilized in calcium alginate, the operational stability during air-lift reactor operation was also found to increase with up to three times longer half-life(44 days) of catalytic activity compared with immobilized intact cells.

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Authors and Affiliations

  1. Applied Microbiology Research Group, Genetic Engineering Research Institute, KIST, P.O. Box 115, 305-600, Yusong, Taejon, Korea

    Young-Min Park, Eui-Sung Choi & Sang-Ki Rhee

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  1. Young-Min Park
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  2. Eui-Sung Choi
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  3. Sang-Ki Rhee
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Park, YM., Choi, ES. & Rhee, SK. Effect of toluene-permeabilization on oxidation of D-sorbitol to L-sorbose by Gluconobacter suboxydans cells immobilized in calcium alginate. Biotechnol Lett 16, 345–348 (1994). https://doi.org/10.1007/BF00245048

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  • DOI: https://doi.org/10.1007/BF00245048

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