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Determination of 3-hydroxytyramine and dopa in various organs of dog after dopa-infusion

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Summary

Some fluorimetrical techniques used for the determination of catecholamines have been studied in order to establish their reliability for determinations in organs after infusion of 3,4-dihydroxyphenylalanine (dopa).

In dogs conversion of infused dopa into 3-hydroxytyramine (dopamine) seems to occur in various organs. The role played by heart, liver, spleen, and kidney in the decarboxylation of exogenic 3,4-dihydroxyphenylalanine is difficult to evaluate. Release of 3-hydroxytyramine into the blood stream or uptake of this substance from the blood could have significantly influenced the results.

Zusammenfassung

Einige Methoden zur fluorimetrischen Bestimmung von Katecholaminen wurden im Hinblick auf ihre Zuverlässigkeit für Bestimmungen in Organen nach Infusion von 3,4-Dihydroxyphenylalanin (Dopa) untersucht.

Bei Hunden erfolgt die Decarboxylierung von Dihydroxyphenylalanin zu 3-Hydroxytyramin (Dopamin) offenbar multiloculär. Die Rolle, die das Herz, die Leber, die Milz und die Nieren bei der Decarboxylierung von exogenem 3,4-Dihydroxyphenylalanin spielen, ist schwer abzuschätzen. Abgabe von 3-Hydroxytyramin in die Blutbahn oder Aufnahme aus dem Blutstrom könnten den Gehalt der Organe an 3-Hydroxytyramin nach Infusion von 3,4-Dihydroxyphenylalanin entscheidend beeinflußt haben.

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  1. A. Wegmann

    Present address: Farbwerke Hoechst, Medizinisch-Wissenschaftliche Abteilung, Frankfurt/M.-Höchst, Germany

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  1. From the Department of Medicine, Wayne State University, Detroit, Michigan, U.S.A.

    A. Wegmann

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Wegmann, A. Determination of 3-hydroxytyramine and dopa in various organs of dog after dopa-infusion. Naunyn - Schmiedebergs Arch 246, 184–190 (1963). https://doi.org/10.1007/BF00245004

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  • DOI: https://doi.org/10.1007/BF00245004

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