Abstract
Acinetobacter calcoaceticus is able to produce a β-lactamase which was found in the periplasm and to be released into the extracellular culture medium. β-Lactamase export was dependent on enzyme over-production in a cooperative manner. Furthermore, it was accompanied by a steadily increasing release of lipopolysaccharide, an outer membrane constituent, and by an increase in the susceptibility to hydrophobic antibiotics. The data point towards a self-promoted perturbation of the outer membrane by overproduction of the enzyme, leading to a semi-selective increase in membrane permeability.
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Abbreviations
- KDO:
-
3-deoxy-d-manno-2-octulosonic acid
- LPS:
-
lipopolysaccharide
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Borneleit, P., Blechschmidt, B., Eschrich, K. et al. β-Lactamase export across the outer membrane of Acinetobacter calcoaceticus:perturbation of the outer membrane lipopolysaccharide leaflet by enzyme overproduction. Arch. Microbiol. 155, 500–504 (1991). https://doi.org/10.1007/BF00244969
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DOI: https://doi.org/10.1007/BF00244969