Abstract
Roseobacter denitrificans, previously named Erythrobacter species OCh 114, synthesized spheroidenone as a major carotenoid under aerobic dark conditions. When the dark-grown cells were subjected to illumination under anacrobic conditions, many unknown yellow pigments appeared and a considerable amount of spheroidenone disappeared. Absorption maxima of these pigments were blue-shifted from those of spheroidenone. The most abundant of the pigments was isolated, and its chemical structure was determined as 3,4-dihydrospheroidenone on spectroscopic and chemical evidence. Presumably, over-reduction of the photosynthetic apparatus interfered with normal photosynthetic electron transfer and resulted in photoreduction of C=C double bond at the 3,4-position of spheroidenone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BChl:
-
bacteriochlorophyll a
- HPLC:
-
high-performance liquid chromatography
- FD-MS:
-
field desorption-mass spectrometry
- 1H-NMR:
-
proton nuclear magnetic resonance
- 2D-COSY:
-
two dimensional correlated spectroscopy
- NOE:
-
nuclear Overhauser effect
References
Arata H, Serikawa Y, Takamiya K (1988) Trimethylamine N-oxide respiration by aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114. J Biochem 103: 1011–1015
Arm C, Pfander H (1984) 1,2-Epoxycarotinoide: 5. Synthese von (S)-1,2-Epoxy-1,2,7,8,7′,8′-hexahydro-ψ,ψ-carotin [(S)-1,2-Epoxy-1,2-dihydro-ζ-carotin]. Helv Chim Acta 67: 1540–1546
Barry P, Young AJ, Britton G (1990) Photodestruction of pigments in higher plants by herbicide action: I. The effect of DCMU (Diuron) on isolated chloroplasts. J Exp Bot 41: 123–129
Harashima K, Hayasaki J, Ikari T, Shiba T (1980) O2-stimulated synthesis of bacteriochlorophyll and carotenoids in marine bacteria. Plant Cell Physiol 21: 1283–1294
Harashima K, Nakagawa M, Murata N (1982) Photochemical activities of bacteriochlorophyll in aerobically grown cells of aerobic heterotrophs, Erythrobacter species (OCh 114) and Erythrobacter longus (OCh 101). Plant Cell Physiol 23: 185–193
Harashima K, Nakada H (1983) Carotenoids and ubiquinone in aerobically grown cells of an aerobic photosynthetic bacterium, Erythrobacter species OCh 114. Agric Biol Chem 47: 1057–1063
Harashima K, Kawazoe K, Yoshida I, Kamata H (1987) Light-stimulated aerobic growth of Erythrobacter species OCh 114. Plant Cell Physiol 28: 365–374
Jackman LM, Liaaen-Jensen S (1964) Bacterial carotenoids: XV. On the constitution of the minor carotenoids of Rhodopseudomonas: 5. The structures of P518 (2,2′-diketospirilloxanthin), OH-R (OH-spheroidenone), and OH-Y (OH-spheroidene). Acta Chem Scand 18: 1403–1411
Ke B, Imsgard F, Kjøsen H, Liaaen-Jensen S (1970) Electronic spectra of carotenoids at 77 K. Biochim Biophys Acta 210: 139–152
Koyama Y, Takatsuka I, Kanaji M, Tomimoto K, Kito M, Shimamura T, Yamashita J, Saiki K, Tsukida K (1990) Configurations of carotenoids in the reaction center and the light-harvesting complex of Rhodospirillum rubrum. Natural selection of carotenoid configurations by pigment protein complexes. Photochem Photobiol 51: 119–128
Lutz M, Szponarski W, Berger G, Robert B, Neumann J-M (1987) The stereoisomerism of bacterial, reaction-center-bound carotenoids revisited: an electronic absorption, resonance Raman and 1H-NMR study. Biochim Biophys Acta 894: 423–433
Noguchi T, Hayashi H, Tasumi M (1990) Factors controlling the efficiency of energy transfer from carotenoids to bacteriochlorophyll in purple photosynthetic bacteria. Biochim Biophys Acta 1017: 280–290
Okamura K, Takamiya K, Nishimura M (1985) Photosynthetic electron transfer system is inoperative in anaerobic cells of Erythrobacter species strain OCh 114. Arch Microbiol 142: 12–17
Shiba T (1991) Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contains bacteriochlorophyll a. Syst Appl Microbiol (in press)
Shioi Y, Doi M, Arata H, Takamiya K (1988) A denitrifying activity in an aerobic photosynthetic bacterium, Erythrobacter sp. strain OCh 114. Plant Cell Physiol 29: 861–865
Straub O (1987) Key to carotenoids, 2nd edn. Birkhäuser, Basel
Takaichi S, Shimada K, Ishidsu J (1988) Monocyclic cross-conjugated carotenal from an aerobic photosynthetic bacterium, Erythrobacter longus. Phytochemistry 27: 3605–3609
Takaichi S, Shimada K, Ishidsu J (1990) Carotenoids from the aerobic photosynthetic bacterium, Erythrobacter longus: β-carotene and its hydroxyl derivatives. Arch Microbiol 153: 118–122
Takamiya K, Arata H, Shioi Y, Doi M (1988) Restoration of the optimal redox state for the photosynthetic electron transfer system by auxiliary oxidants in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114. Biochim Biophys Acta 935: 26–33
Weedon BCL (1969) Spectroscopic methods for elucidating the structures of carotenoids. Fortschr Chem Org Naturst 27: 81–130
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takaichi, S., Furihata, K. & Harashima, K. Light-induced changes of carotenoid pigments in anaerobic cells of the aerobic photosynthetic bacterium, Roseobacter denitrificans (Erythrobacter species OCh 114): reduction of spheroidenone to 3,4-dihydrospheroidenone. Arch. Microbiol. 155, 473–476 (1991). https://doi.org/10.1007/BF00244964
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00244964