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Purification and characterization of a surface-associated carotenoid-binding complex from the photosynthetic prokaryote, Prochlorothrix hollandica

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Abstract

We have identified a water-soluble surface-associated complex from Prochlorothrix hollandica, composed of two polypeptides of 56 and 58 kilodaltons (kDa), zeaxanthin, and lipopolysaccharide. The complex was purified by preparative isoelectric focusing (pI=3.0). The outer membrane lipopolysaccharide co-purified with the complex. Immunocytochemisty employing a polyclonal antibody to the apoproteins exclusively labeled the cell surface. Both zeaxanthin and the protein accumulated under high light intensities, thus we propose that the complex may play a role in photoprotection.

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Engle, J.M., Burkhart, W., Sherman, D.M. et al. Purification and characterization of a surface-associated carotenoid-binding complex from the photosynthetic prokaryote, Prochlorothrix hollandica . Arch. Microbiol. 155, 453–458 (1991). https://doi.org/10.1007/BF00244961

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