Abstract
Electroporation methods and conjugal matings were used to transfer several plasmid vectors to Aquaspirillum dispar and Aquaspirillum itersonii. The incompatibility P class plasmid RP4 was conjugally transferred from Escherichia coli HB101 to these spirilla, and the transconjugants subsequently donated the molecule to plasmid-free E. coli and A. dispar strains via conjugal matings. High-voltage electrotransformation was used to transfer plasmids pUCD2, pSa151 and RP4 to A. dispar and A. itersonii, at efficiencies as high as 3×104 transformants per μg plasmid DNA. RP4 DNA isolated from spirillum hosts, but not RP4 from E. coli cells was successfully transferred to A. dispar and A. itersonii by electrotransformation, suggesting that modification and/or restriction activity may be present in these Aquaspirillum species.
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References
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513–1523
Blakemore RP, Frankel RB, Wolfe RS (1980) Magnetite in freshwater magnetotactic bacteria. Science 203: 1355–1356
Blakemore RP, Maratea D, Wolfe RS (1979) Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. J Bacteriol 140: 720–729
Close TJ, Zaitlin D, Kado CI (1984) Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58
Datta N, Hedges RW (1972) Host ranges of R factors. J Gen Microbiol 70: 453–460
Holloway BW (1978) Plasmids that mobilize bacterial chromosome. Plasmid 2: 1–19
Krieg NR (1984) Genus Aquaspirillum. In: Holt JG, Mair NS, Sharpe EM, Sneath PHA (eds) Bergeys manual of systematic bacteriology, vol. I. The Williams & Wilkins Co., Baltimore, pp 71–90
Krieg NR (1981) The genus Aquaspirillum. In: Balows A, Schlegel HG, Starr MP, Trüper HG (eds) The prokaryotes, vol. II. Springer, New York, pp 595–608
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Miller JF, Dower WJ, Tompkins LS (1988) High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. Proc Natl Acad Sci USA 85: 856–860
Shigekawa K, Dower WJ (1988) Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells. Biotechniques 68: 742–751
Tait RC, Lundquist RC, Kado CI (1982) Genetic map of the crown gall suppressive IncW plasmid pSa. Mol Gen Genet 186: 10–15
Waleh NS (1988) Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12. Mol Gen Genet 214: 592–594
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Eden, P.A., Blakemore, R.P. Electroporation and conjugal plasmid transfer to members of the genus Aquaspirillum . Arch. Microbiol. 155, 449–452 (1991). https://doi.org/10.1007/BF00244960
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DOI: https://doi.org/10.1007/BF00244960