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Carbonic anhydrase activity of Prochloron sp. isolated from an ascidian host

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Abstract

The prokaryotic algal symbiont of ascidians, Prochloron sp., was found to exhibit carbonic anhydrase activity which is largely associated with the cell surface. This extracellular carbonic anhydrase activity was inhibited, while the intracellular activity was not affected, by chloride or bromide. Acetazolamide and ethoxyzolamide inhibited carbonic anhydrase activity with I50 values of 7×10-4 and 3×10-4M, respectively. These I50 values are similar to those observed for intracellular carbonic anhydrases of Synechococcus sp. PCC7942, Chlamydomonas reinhardii and spinach.

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Abbreviations

AZA:

acetazolamide

CA:

carbonic anhydrase

chl:

chlorophyll

EZA:

ethozyzolamide

I50 :

concentration of an inhibitor required to cause 50% inhibition

Rubisco:

ribulose-1,5-bisphosphate carboxylase/oxygenase

U:

unit

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Authors and Affiliations

  1. Marine Biotechnology Institute, Shimizu Laboratory, 1900 Sodeshi-cho, 424, Shimizu-shi, Shizuoka, Japan

    Maribel L. Dionisio-Sese, Atsuhiro Shimada & Tadashi Maruyama

  2. Marine Biotechnology Institute, Head Office, 2-35-10 Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Shigetoh Miyachi

Authors
  1. Maribel L. Dionisio-Sese
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  2. Atsuhiro Shimada
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  3. Tadashi Maruyama
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  4. Shigetoh Miyachi
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Dionisio-Sese, M.L., Shimada, A., Maruyama, T. et al. Carbonic anhydrase activity of Prochloron sp. isolated from an ascidian host. Arch. Microbiol. 159, 1–5 (1993). https://doi.org/10.1007/BF00244256

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  • DOI: https://doi.org/10.1007/BF00244256

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