Abstract
A strategy is presented for fixing regulated transport systems in the functional or non-functional state. The advantages of this approach include the ability to distinguish between sensor and effector systems, the capacity to study transporters independently of their activating mechanisms, and the possibility of identifying membrane polypeptides that participate in the regulatory process.
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Parker, J.C., Glosson, P.S. & Walstad, D.L. Fixation of transporters in the active or inactive state. Mol Cell Biochem 82, 91–95 (1988). https://doi.org/10.1007/BF00242522
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DOI: https://doi.org/10.1007/BF00242522