Summary
At the concentration needed for NMR, the calcium-saturated form of calcineurin B dissolved in water shows resonance line widths that indicate aggregation of this protein. Although the line width or aggregation state can be influenced to some degree by temperature, pH, and salt concentrations, in the absence of detergent no conditions could be found where the protein behaved as a monomeric unit. In the presence of a 10- to 20-fold molar excess of the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propanesulfonate (CHAPS), resonance line widths were considerably narrower and were compatible with a protein of ∼25 kDa. The presence of the NMR signals of the non-deuterated CHAPS does not interfere with modern isotope-directed NMR studies as the signals from protons not attached to 15N or 13C are removed by isotope filtering and purge pulses.
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Anglister, J., Grzesiek, S., Ren, H. et al. Isotope-edited multidimensional NMR of calcineurin B in the presence of the non-deuterated detergent CHAPS. J Biomol NMR 3, 121–126 (1993). https://doi.org/10.1007/BF00242480
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DOI: https://doi.org/10.1007/BF00242480