Summary
The identification of somatic hybrids between Solanum tuberosum and S. brevidens can be carried out using polymerase chain reaction (PCR) and arbitrary 10-mer primers to generate random amplified polymorphic DNA (RAPD) markers. Five commercial primers have been tested. Each primer directed the amplification of a genome-specific “fingerprint” for the fusion parents and S. brevidens. The size of the amplified DNA fragments ranged from 100 to 1800 base pairs. The somatic hybrids showed a combination of the parental banding profiles with four of the five primers surveyed, whereas regenerants from one of the parents had the same or a similar banding pattern to that of the parent. Thus RAPD markers provide a quick, simple and preliminary screening method for putative somatic hybrids.
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Abbreviations
- EDTA:
-
ethylenediaminetetraacetic acid,
- PCR:
-
polymerase chain reaction
- RAPD:
-
random amplified polymorphic DNA
- RFLP:
-
restriction fragment length polymorphisms
- TBE:
-
Tris-borate-EDTA buffer
- Tris:
-
trizma base
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Xu, Ys., Clark, M.S. & Pehu, E. Use of RAPD markers to screen somatic hybrids between Solanum tuberosum and S. brevidens . Plant Cell Reports 12, 107–109 (1993). https://doi.org/10.1007/BF00241944
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DOI: https://doi.org/10.1007/BF00241944