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Establishment of fast-growing callus and root cultures of Cephalotaxus harringtonia

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Summary

Callus cultures were established from Cephalotaxus harringtonia (Japanese plumyew) stem expiants cultured on Murashige and Skoog medium supplemented with 4.5 μM 2,4-dichlorophenoxyacetic acid and 0.05 βM 6-furfurylaminopurine. The inclusion of 4.9 μM 6-(γ,γ-dimethylallylamino) purine as the sole hormone significantly increased the growth rate of the callus. Organogenesis giving rise to both shoots and roots occurred upon transfer of the callus onto a hormonefree medium. Vitrification was common on all regenerated shoots cultured on Gelrite-containing medium. Regenerated roots were excised and established in McCown's woody plant medium. Doubling the phosphate and nitrate levels in the medium increased the growth of these root cultures.

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Abbreviations

MS:

Murashige and Skoog basal medium

B5:

Gamborg's B5 basal salt medium

WP:

McCown's woody plant basal salt medium

2,4-D:

2,4-dichlorophenoxyacetic acid

Kinetin:

6-furfurylamino-purine

2iP:

6-(γ,γ-dimethylallylamino) purine

IBA:

indole-3-butyric acid

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Authors and Affiliations

  1. Department of Horticulture, The Pennsylvania State University, 16802, University Park, PA, USA

    Enaksha R. Wickremesinhe & Richard N. Arteca

Authors
  1. Enaksha R. Wickremesinhe
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  2. Richard N. Arteca
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Communicated by J. J. Finer

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Wickremesinhe, E.R., Arteca, R.N. Establishment of fast-growing callus and root cultures of Cephalotaxus harringtonia . Plant Cell Reports 12, 80–83 (1993). https://doi.org/10.1007/BF00241939

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  • DOI: https://doi.org/10.1007/BF00241939

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