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Somatic embryogenesis and plant regeneration from immature cotyledons of watermelon

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Abstract

Cotyledon expiants from immature embryos of five watermelon [Citrullus lanatus (Thunb.)Matsum. & Nakai] genotypes were incubated in the dark for three weeks on a modified MS medium containing B5 vitamins, 2,4-D (10, 20 or 40μM), 0.5 μM of either BA or TDZ, and 7 g·1-1 TC agar. Somatic embryos, some with well developed cotyledons, were observed on cotyledon expiants three to four weeks after transfer to MS medium without PGRs and 16h photoperiod. The best PGR combination for somatic embryogenesis was 10 μM 2,4-D and 0.5 μM TDZ Somatic embryogenesis was greatest (30%) when cotyledon expiants were established from 18-day-old immature embryos. Somatic embryos were germinated on MS medium without PGRs. Plants were transferred to Magenta boxes containing ProMix for three weeks before being transplanted to the field where they formed fertile male and female flowers that produced normal fruit.

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Abbreviations

PGR:

plant growth regulator

BA:

benzyladenine

TDZ:

thidiazuron

2,4-D:

2,4-dichlorophenoxyaceticacid

NAA:

α-naphthaleneacetic acid

2,4,5-T:

2,4,5-trichlorophenoxyacetic acid

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Authors and Affiliations

  1. Central Florida Research and Education Center, University of Florida, Institute of Food and Agricultural Sciences, 5336 University Ave., 34748, Leesburg, Florida, USA

    Michael E. Compton & D. J. Gray

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  1. Michael E. Compton
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  2. D. J. Gray
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Communicated by J. J. Finer

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Compton, M.E., Gray, D.J. Somatic embryogenesis and plant regeneration from immature cotyledons of watermelon. Plant Cell Reports 12, 61–65 (1993). https://doi.org/10.1007/BF00241935

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  • DOI: https://doi.org/10.1007/BF00241935

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