Summary
Inoculation of large-scale plant root culture reactors can be carried out by briefly homogenizing bulk root tissue, followed by aseptic transfer as a slurry to the reactor. Uniform root distribution can be achieved in bioreactors by entrapment of the growing root inoculum onto process packing elements (e.g. distillation packings), randomly distributed within the reactor, in a bubble column operation. These two techniques have been successfully used to inoculate a 14 L pilot-scale reactor which is subsequently operated as a trickle bed reactor.
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References
Curtis, W.R., (1993). Current Opinion in Biotechnology, 4:205–210.
Charlwood, B.V., Charlwood, K.A. and Molina-Torres, J. (1990). In Secondary Products from Plant Tissue Culture, Charlwood, B.V. and Rhodes, M.J.C., eds. 167–200, Oxford: Clarendon Press.
Dilorio, A.A., Cheetham, R.D. and Weathers, P.J., (1992). Appl. Microbiol. Biotechnol., 37, 457–462.
Doran, P.M. and Sharp, J.M. (1990). J. Biotechnol., 16, 171–186.
Dunlop, D.S. and Curtis, W.R. (1991). Biotechnol. Prog., 7, 434–438.
Flores, H.E. and Curtis, W.R., (1992). In Biochemical Engineering VII, Pederson, H., Mutharasan, R. and Di Biasio, D., eds. 188–209, New York: The New York Academy of Sciences.
McKelvey, S.A., Gehrig, J.A., Hollar, K.A. and Curtis, W.R. (1993). Biotechnol. Prog., 9, 317–322.
Payne, G.F., Bringi, V., Prince, C. and Shuler, M.L. (1991) Plant Cell and Tissue Culture in Liquid Systems, Munich: Hanser Publishers.
Ramakrishnan, D. and Curtis, W.R. (1994). In Advances in Plant Biotechnology: Production of Secondary Metabolites, Furusaki, S., Ryu, D., eds. 281–305, Amsterdam: Elseviers.
Rhodes, M.J.C., Hilton, M., Parr, A.J., Hamill, J.D. and Robins, R.J. (1986). Biotechnol. Lett., 8, 415–420.
Shah, Y.T., (1979). Gas-Liquid-Solid Reactor Design. New York: McGraw Hill.
Taya, M., Yoyama, A., Kondo, O.,Kobayashi, T. and Matsui, C., (1989). J. Chem. Eng. Japan, 22 (1), 84–89.
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Ramakrishnan, D., Salim, J. & Curtis, W.R. Inoculation and tissue distribution in pilot-scale plant root culture bioreactors. Biotechnol Tech 8, 639–644 (1994). https://doi.org/10.1007/BF00241690
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DOI: https://doi.org/10.1007/BF00241690