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The radiosensitivity of uveal melanoma cells and the cell survival curve

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Abstract

• Background: No study of the radiosensitivity of uveal melanoma cells and their survival curve has been published. The purpose of this study was to investigate the sensitivity to different single radiation doses of SP6.5, a human uveal melanoma cell line. • Methods: Cells were irradiated with cobalt-60 at doses from 0 to 1200 cGy. Radiosensitivity was measured by three methods: soft-agar bilayer assay, tritiated thymidine incorporation, and bromodeoxyuridine (BrdU) incorporation. • Results: The soft-agar bilayer assay, by assessing the colony-forming units, showed that the D 1 value was 470 cGy, the D q value was 400 cGy, and the n value exceeded 10, thus indicating a broad, shoulder and relative radioresistance. The doubling time as estimated by [3H]thymidine incorporation was unaffected at doses below 600 cGy, another indication of radioresistance. BrdU incorporation revealed no significant increase between 0 and 1000 cGy, indicating that the cell cycle was not interrupted. • Conclusion: Cell survival, doubling time, and cell phases are parameters of growth kinetics, and the results suggest that SP6.5 is radioresistant and virtually unaffected by single radiation doses lower than 600 cGy. Our data parallel published data for cutaneous melanomas.

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Authors and Affiliations

  1. Centre de recherche en inflammation, immunologic et rhumatologie, Centre de recherche du CHUL, Department of Ophthalmology, Faculty of Medicine, Laval University and Centre Hospitalier de l'Université Laval, 2705 Boulevard Laurier, GIV 4G2, Ste Foy, Quebec, Canada

    Denis Soulières, Alain Rousseau, Manon Tardif, Luc Vaillancourt & Guy Pelletier

  2. Department of Radiotherapy, Hôtel-Dieu de Quebec, Quebec, Canada

    Marie Larochelle

  3. Department of Pathology, CHUL, Ste Foy, Quebec, Canada

    Michel Tremblay

Authors
  1. Denis Soulières
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  2. Alain Rousseau
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  3. Manon Tardif
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  4. Luc Vaillancourt
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  5. Guy Pelletier
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  6. Marie Larochelle
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  7. Michel Tremblay
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Soulières, D., Rousseau, A., Tardif, M. et al. The radiosensitivity of uveal melanoma cells and the cell survival curve. Graefe's Arch Clin Exp Ophthalmol 233, 85–89 (1995). https://doi.org/10.1007/BF00241477

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  • DOI: https://doi.org/10.1007/BF00241477

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