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Planta

, Volume 191, Issue 1, pp 79–85 | Cite as

Characterization of the plasma-membrane-bound nitrate reductase in Chlorella saccharophila (Krüger) Nadson

  • Christine Stöhr
  • Rudolf Tischner
  • Michael R. Ward
Article

Abstract

The plasma membranes of Chlorella saccharophila (Krüger) Nadson cells contained a membrane-bound nitrate reductase. This form of nitrate reductase was purified and characterized. Several differences from the soluble form of nitrate reductase were apparent, the most important being: (i) the greater hydrophobicity, as proven using Triton X-114 phase separation, hydrophobic interaction chromatography and stimulation by phosphilipids; (ii) the differences in the native molecular mass compared with Chlorella sorokiniana (Krüger) Nadson; and (iii) the different polypeptide pattern obtained by two-dimensional polyacrylamide gel electrophoresis. Only the plasma-membrane-bound nitrate reductase could be found in both inside-out and right-side-out plasma-membrane vesicles.

Key words

Chlorella Nitrate reductase (plasma-membrane bound) Plasma membrane 

Abbreviations

HIC

hydrophobic interaction chromatography

IEF

isoelectric focusing

MV

methyl viologen

NR

nitrate reductase

PM

plasma membrane

PMNR

plasma-membrane-bound nitrate reductase

SDS-PAGE

sodium dodecyl sulfatepolyacrylamide gel electrophoresis

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Christine Stöhr
    • 1
  • Rudolf Tischner
    • 1
  • Michael R. Ward
    • 2
  1. 1.Pflanzenphysiologisches Institut der Universität GöttingenGöttingenGermany
  2. 2.Department of Molecular BiologyMassachusetts General HospitalBostonUSA

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