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Mechanism of protein synthesis inhibition during stationary phase in Bacillus stearothermophil US

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Summary

The appearance of a protein (association factor I) in ribosomes from Bacillus stearothermophilus at stationary phase of growth is described. Association factor I is present on 30S subunits and 30S–50S ribosomal couples, but not on 50S subunits. This protein is responsible for the low levels of polyphenylalanine synthesis shown by stationary phase ribosomes. Association factor I is able to bind to free 30S–50S ribosomal couples but not to polysomes, and exerts its effect by inhibiting the initiation step of protein synthesis. Ribosomes preincubated with association factor I have a decreased ability for polypeptide snythesis directed phage mRNA or poly(U).

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References

  1. Li, L. and Umbreit, W. W., 1966. Biochim. Biophys. Acta 119, 392–399.

    Google Scholar 

  2. cheps, R., Wax, R. and Revel, M., 1971. Biochim. Biophys. Acta 232, 140–150.

    Google Scholar 

  3. González, N. S., Goldemberg, S. H. and Algranati, I. D., 1968. Biochim. Biophys. Acta 166, 760–762.

    Google Scholar 

  4. Ramagropal, S. and Subramanian, A. R., 1975. J. Mol. Biol. 94, 633–641.

    Google Scholar 

  5. Subramanian, A. R., Haase, C. and Giesen, M., 1976. Eur. J. Biochem. 67, 591–601.

    Google Scholar 

  6. García-Patrone, M., 1978. FEBS Lett. 92, 263–267.

    Google Scholar 

  7. García-Patrone, M., González, N. S. and Algranati, I. D., 1975. Biochim. Biophys. Acta 395, 373–380.

    Google Scholar 

  8. Algranati, I. D., González, N. S. and Bade, E. G., 1969. Proc. Natl. Acad. Sci. USA 62, 574–580.

    Google Scholar 

  9. Bade, E. G., González, N. S. and Algranati, I. D., 1969. Proc. Natl. Acad. Sci. USA 64, 654–660.

    Google Scholar 

  10. Lodish, H. F. and Robertson, H. G., 1969. Cold Spring Harbor Symp. Quant. Biol. XXXIV, 655–673.

    Google Scholar 

  11. Kay, A. C., Graffe, M. and Crumberg-Manago,., 1976. Biochimie 58, 183–199.

    Google Scholar 

  12. Schreier, M. H. and Noll, H., 1970. Nature 227, 128–133.

    Google Scholar 

  13. Ono-Iwashita, Y., Oshima, T. and Imahori, K., 1975. Arch. Biochem. Biophys. 171, 490–499.

    Google Scholar 

  14. Igarashi, K., Hara, K., Watanabe, Y., Hirose, S. and Takeda, Y., 1975. Biochem. Biopbys. Res. Commun. 64, 897–904.

    Google Scholar 

  15. Studier, F. W., 1973. J. Mol. Biol. 79, 237–248.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Instituto de Investigaciones Bioquímicas “Fundación Campomar” and Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428, Buenos Aires, Argentina

    M. Garcia-Patrone

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  1. M. Garcia-Patrone
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Garcia-Patrone, M. Mechanism of protein synthesis inhibition during stationary phase in Bacillus stearothermophil US . Mol Cell Biochem 31, 97–103 (1980). https://doi.org/10.1007/BF00240815

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  • DOI: https://doi.org/10.1007/BF00240815

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