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Effects of estradiol-17β in the male xenopus laevis: Isolation and translation of cytoplasmic messenger rna populations

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Summary

Total Xenopus liver cytoplasmic RNA isolated following long-term estrogen administration (14 days) was fractioned using Sepharose 4B chromatography. One of the Sepharose 4B peaks was shown to contain RNA with a molecular weight reported for vitellogenin mRNA (∼34S). The presence of estrogeninduced vitellogenin mRNA in the peak 5 RNA was determined by translation of the RNA in the oocyte and analysis of the oocyte translational products by immunoprecipitation with anti-vitellogenin.

Sepharose 4B peaks 2 and 3 were also observed to contain estrogen induced mRNA populations sedimenting between 9-18S. These findings suggest that Sepharose 4B chromatography might prove useful in separating different mRNA populations following estrogen-induced gene activation.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Medical College of Georgia, 30901, Augusta, Georgia, USA

    Thomas L. Wiggins III, Linda M. Tucciarone & Kenneth D. Lanclos

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  1. Thomas L. Wiggins III
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  2. Linda M. Tucciarone
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  3. Kenneth D. Lanclos
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Wiggins, T.L., Tucciarone, L.M. & Lanclos, K.D. Effects of estradiol-17β in the male xenopus laevis: Isolation and translation of cytoplasmic messenger rna populations. Mol Cell Biochem 21, 145–151 (1978). https://doi.org/10.1007/BF00240133

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