Abstract
Nitrogen-starved sunflower plants (Helianthus annuus L. cv. Peredovic) cannot absorb NO −3 or NO −2 upon initial exposure to these anions. Ability of the plants to take up NO −3 and NO −2 at high rates from the beginning was induced by a pretreatment with NO −3 . Nitrite also acted as inducer of the NO −2 -uptake system. The presence of cycloheximide during NO −3 -pretreatment prevented the subsequent uptake of NO −3 and NO −2 , indicating that both uptake systems are synthesized de novo when plants are exposed to NO −3 . Cycloheximide also suppressed nitrate-reductase (EC 1.6.6.1) and nitrite-reductase (EC 1.7.7.1) activities in the roots. The sulfhydryl-group reagent N-ethylmaleimide greatly inhibited the uptake of NO −3 and NO −2 . Likewise, N-ethylmaleimide promoted in vivo the inactivation of nitrate reductase without affecting nitrite-reductase activity. Rates of NO −3 and NO −2 uptake as a function of external anion concentration exhibited saturation kinetics. The calculated Km values for NO −3 and NO −2 uptake were 45 and 23 μM, respectively. Rates of NO −3 uptake were four to six times higher than NO −3 -reduction rates in roots. In contrast, NO −2 -uptake rates, found to be very similar to NO −3 -uptake rates, were much lower (about 30 times) than NO −2 -reduction rates. Removal of oxygen from the external solution drastically suppressed NO −3 and NO −2 uptake without affecting their reduction. Uptake and reduction were also differentially affected by pH. The results demonstrate that uptake of NO −3 and NO −2 into sunflower plants is mediated by energy-dependent inducible-transport systems distinguishable from the respective enzymatic reducing systems.
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Abbreviations
- CHI:
-
cycloheximide
- NEM:
-
N-ethylmaleimide
- NiR:
-
nitrite reductase
- NR:
-
nitrate reductase
- pHME:
-
p-hydroxymercuribenzoate
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This research was supported by grant PB86-0232 from the Dirección General de Investigatión Científica y Técnica (Spain). One of us (E.A.) thanks the Consejeria de Educación y Ciencia de la Junta de Andalucia for the tenure of a fellowship. We thank Miss G. Alcalá and Miss C. Santos for their valuable technical and secretarial assistance.
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Agüera, E., de la Haba, P., Fontes, A.G. et al. Nitrate and nitrite uptake and reduction by intact sunflower plants. Planta 182, 149–154 (1990). https://doi.org/10.1007/BF00239997
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DOI: https://doi.org/10.1007/BF00239997