Abstract
Tomato (Solanum lycopersicon L.) contains two B-type phytochrome genes (PHYB1 and PHYB2). Fragments of these two PHYB were cloned following amplification by the polymerase chain reaction of a portion of their relatively well conserved 5′ coding regions. Polypeptides encoded by these gene fragments exhibit 90% sequence identity. These two PHYB are independently expressed in organ-specific fashion. In mature plants, PHYB2 mRNA is most abundant in fruit and PHYB1 mRNA in expanded leaves. A phylogenetic analysis fails to establish which tomato PHYB is orthologous to either Arabidopsis PHYB or PHYD, the latter being a second B-type phytochrome. Instead, this analysis indicates that following the divergence of the Solanaceae and Brassicaceae from one another, a PHYB gene duplicated independently in each lineage. Consequently, Arabidopsis PHYB mutants cannot be considered strictly equivalent to the tomato tri mutants, which appear to be mutated at the PHYB1 locus. Similarly, other putative PHYB mutants might not be equivalent to those described for Arabidopsis and tomato. This situation complicates efforts to determine ‘PHYB function’ because there might be no one answer to this question.
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Abbreviations
- PCR:
-
polymerase chain reaction
- PHY :
-
undesignated phytochrome gene
- PHYA, PHYB, etc:
-
phytochrome gene(s) of the A, B, etc. type
References
Cherry JR, Vierstra RD (1994) The use of transgenic plants to examine phytochrome structure/function. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants, 2nd edn. Kluwer, Dordrecht, The Netherlands, pp 271 -297
Clack T, Mathews S, Sharrock RA (1994) The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE. Plant Mol Biol 193:413–427
Cordonnier-Pratt M-M, Pratt LH, Hauser B, Kodiert G, Caboche M (1994) Comparative analysis of the phytochrome gene family in tomato (Lycopersicon esculentum Mill.) and sorghum (Sorghum bicolor [L.] Moench). (Abstract) Plant Physiol 105 [Suppl]:72
Dehesh K, Tepperman J, Christensen AH, Quail PH (1991) phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet 225:305–313
Devereux J (1989) The GCG sequence analysis software package, version 6.0. University Research Park, Madison, Wis., USA
Heyer A, Gatz C (1992) Isolation and characterization of a cDNA-clone coding for potato type B phytochrome. Plant Mol Biol 20:589–600
Kendrick RE, Kerckhoffs LHJ, Pundsnes AS, Van Tuinen A, Koornneef M, Nagatani A, Terry MJ, Tretyn A, Cordonnier-Pratt M-M, Hauser B, Pratt LH (1994) Photomorphogenic mutants of tomato. Euphytica 79:227–234
Kerckhoffs LHJ, Van Tuinen A, Nagatani A, Koornneef M, Kendrick RE (1994) Temporarily red light-insensitive mutants of tomato. In: Abstracts of 4th International Congress of Plant Molecular Biology, Amsterdam, abstract 1063
Kern R, Gasch A, Deak M, Kay SA, Chua N-H (1993) PhyB of tobacco, a new member of the phytochrome family. Plant Physiol 102:1363–1364
Koornneef M, Kendrick RE (1994) Photomorphogenic mutants of higher plants. In:Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants, 2nd edn. Kluwer, Dordrecht, The Netherlands, pp 601–628
Kumar S, Tamura K, Nei M (1993) MEGA: molecular evolutionary genetics analysis, version 1.0. The Pennsylvania State University, University Park, Pa., USA
Mathews S, Lavin M, Sharrock RA (1995) Evolution of the phytochrome gene family and its utility for phylogenetic analyses of angiosperms. Ann Mo Bot Gard 82:296–321
Meyer C, Pouteau S, Rouzé P, Caboche M (1994) Isolation and molecular characterization of dTnp1, a mobile and defective transposable element of Nicotiana plumbaginifolia. Mol Gen Genet 242:194–200
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Pratt LH (1995) Phytochromes: differential properties, expression patterns and molecular evolution. Photochem Photobiol 61: 10–21
Quail PH (1994) Phytochrome genes and their expression. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants, 2nd edn. Kluwer, Dordrecht, The Netherlands, pp 71–104
Quail PH, Boylan MT, Parks BM, Short TW, Xu Y, Wagner D (1995) Phytochromes: photosensory perception and signal transduction. Science 268:675–680
Sharrock RA, Quail PH (1989) Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev 3:1745–1757
Spooner DM, Anderson GJ, Jansen RK (1993) Chloroplast DNA evidence for the interrelationships of tomatoes, potatoes, and pepinos (Solanaceae). Am J Bot 80:676–688
Swofford DL (1993) PAUP — a computer program for phylogenetic inference using maximum parsimony. (abstract) J Gen Physiol 102:A9
Whitelam GC, Harberd NP (1994) Action and function of phytochrome family members revealed through the study of mutant and transgenic plants. Plant Cell Environ 17:615–625
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This research was supported by USDA NRICGP grant 93-00939 and by NATO travel grant CRG 931183. It was initiated when two of us (L.H.P., M.-M.C.-P.) spent a sabbatical year at the Institut National de la Recherche Agronomique in Versailles, France. L.H.P. gratefully acknowledges support provided by a senior guest fellowship from the Ministère de l'enseignement superieur et de la recherche during his stay in Versailles. L.H.P. and M.-M.C.-P thank all of their colleagues in Versailles for their warm hospitality and their willingness to share their expertise with us. We also thank Russell Malmberg, Richard Meagher and Robert Price for helpful discussions concerning the interpretation of molecular phylogenies.
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Pratt, L.H., Cordonnier-Pratt, MM., Hauser, B. et al. Tomato contains two differentially expressed genes encoding B-type phytochromes, neither of which can be considered an ortholog of Arabidopsis phytochrome B. Planta 197, 203–206 (1995). https://doi.org/10.1007/BF00239958
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DOI: https://doi.org/10.1007/BF00239958