Summary
Production of microspore-derived embryos from cultured anthers is now a well established technique for the isolation of homozygous lines in many crop plants. We describe here a culture method for embryo induction and plant regeneration from anthers of four sunflower genotypes. For preliminary experiments, anthers of uninucleate microspores were cultured on four types of basal media viz., Murashige and Skoog's MS, Gamborg's B5, Nitsch and Nitsch, and White's W, supplemented with 1.0 mg/l 2,4 dichlorophenoxy acetic acid and 0.5 mg/l 6-benzylaminopurine and 40 g/l sucrose. MS basal medium, being more responsive for embryo induction, was used for further experimentation. To optimise the culture requirement MS basal medium was supplemented with 0.2–2.0 mg/l 2,4 dichlorophenoxy acetic acid and 0.5 and 1.0 mg/l 6-benzylaminopurine. The effect of cold pretreatment, hormone regime and sucrose concentration were tested for embryogenic efficiency. Genotype had a significant effect on the capacity of embryo induction. Addition of silver nitrate (2.5 mg/l), an ethylene inhibitor, stimulated embryo germination. Plantlets were obtained (10–15%) from embryos of only one genotype.
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Abbreviations
- 2,4-D:
-
2,4 dichlorophenoxy acetic acid
- NAA:
-
α-naphthalene acetic acid
- IAA:
-
indole-3-aceticacid
- BAP:
-
6-benzylaminopurine
- KN:
-
Kinetin
- ABA:
-
abscisic acid
- GA3 :
-
gibberellic acid
References
Brown DCW and Antanassov H (1985) Plant Cell Tiss Org Cult 4:119–122
Chien YE and Kao KN (1983) Can J Bot 61:639–641
Cho Uh-Hiang and Kasha KJ (1989) Plant Cell Rep 8:415–417
Chu CC, Hill RD and Brule-Babel AL (1990) Plant Sci 66:255–262
Dunwell JM (1979) J Exp Bot 30:419–428
Dunwell JM, Cornish M and Courel AGLDE (1985) J Exp Bot 36:679–689
Dunwell JM, Francis RJ and Powell W (1987) Theor Appl Genet 74:60–64
Dunwell JM and Thurling N (1985) J Exp Bot 36:1478–1491
Espinasse A, Charles Lay and Vilin J (1989) Plant Cell Tiss Org Cult 17:171–181
Fienberg AA, Choi JH Lubich WB and Sung ZR (1984) Planta 162:532–539
Finer JJ (1987) Plant Cell Rep 6:372–374
Foroughi-Wehr B, Friedt W and Wenzel G (1982) Theor Appl Genet 62:233–239
Gamborg OL, Miller RA and Ojima K (1968) Exp Cell Res 50:151–158
Gelebart P and San LH (1987) Agronomie (Paris) 7:81–86
Greco B, Tanzarella OA Carrozzo G and Blanco A (1984) Plant Sci 36:73–77
Guerel A, Nichterlein K and Friedt WL (1991) Plant Breed 106:68–76
Keller WA, Arnison PG and Cardy BJ (1987) In: Green CE, Somers DA, Hackelt WP and Bieshoer DD (eds) Plant Tissue and Cell Culture. Alan R Liss Inc., New York, Vol 3, pp 223–241
Kurz SM and Linberger RD (1983) J Am Soc Hort Sci 108:710–714
Lazzeri PA, Hilderbrandt DF and Collins GB (1987) Plant Cell Tiss Org Cult 10:197–208
Maheswari SC, Rashid A and Tyage AK (1982) Am J Bot 69:865–879
Miah MAA, Earle ED and Khush GS (1985) Theor Appl Genet 70:113–116
Murashige T and Skoog F (1962) Physiol Plant 15:473–497
Nitsch JP and Nitsch C (1969) Science 163:85–87
Patterson KE and Everett NP (1985) Plant Sci 42:125–132
Power CJ (1987) Am J Bot 74:497–503
Rines HW (1983) Crop Sci 23:268–272
Rouston JP, Latche A and Fallot J (1990) Plant Sci 67:89–95
Sharma GS, Bello LL and Sapru VT (1980) Euphytica 29:751–754
Sunderland N, Huang B and Hill GJ (1984) J Exp Bot 35:251
Thengane SR, Shrikhande MS and Mascarenhas AF (1990) Fifth Int Youth Course Conf in Genet 11–14 Sept, Albena, Bulgaria
Vasiljevic L, Atlagic J and Skoric D (1990) Ann Biol (Ludhiana) 6:147–152
White PR (1963) In: The Culture of Animal and Plant Cells, The Ronald Press Co., New York, pp59
Yan H, Wu Y Chen X, Wei Z-Y, Zhou C and Yang H-Y (1985) Acta Bot Sin 27:13–18
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Communicated by M. R. Davey
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Thengane, S.R., Joshi, M.S., Khuspe, S.S. et al. Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Reports 13, 222–226 (1994). https://doi.org/10.1007/BF00239897
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DOI: https://doi.org/10.1007/BF00239897