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Ganglioside-cholera toxin interactions: a binding and lipid monolayer study

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Summary

On t.l.c. plates 125I-cholera toxin binds to a disialoganglioside tentatively identified as GDlb with about 10 times less capacity than to ganglioside GM1. Binding of labeled toxin to both gangliosides was abolished in presence of excess amounts of unlabeled B subunit. Ganglioside extracts from human or pig intestinal mucosa showed toxin binding to gangliosides GM1 and GD1b. In ganglioside-containing lipid monolayers the penetration of the toxin was independent of the ganglioside binding capacity.

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Abbreviations

GM2 :

Gal-NAcβ1→4Gal(3←-2αNeuAc)β1←4G1cβ→1Cer

GM1 :

Galβ→3Ga1-NAcβ1→4Gal(3←2αNeuAc)β1→4G1cβ1→1Cer

GD1a :

NeuAcα2→3Ga1→13Gal-NAcβ1→4Gal(3←2αNeuAc)β1→4G1cβ1→1Cer

GD1b:

Galβl→3Gal-NAcβl→4Gal(3←2αNeuAc8←2αNeuAc)β1→4Glcβ1→1Cer

GT1b :

NeuAcα2→3Ga1β1→3Ga1-NAcaβl→4Gal(3←-2αNeuAc8←2αNeuAc)β1→4G1cβ1→1Cer

dpPC:

1,2-hexadecanoyl-sn-glycero-3-phosphocholine

dpPE:

1,2-hexadecanoyl-sn-glycero-3-phosphoethanolamine

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Authors and Affiliations

  1. Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina

    F. A. Cumar, B. Maggio & R. Caputto

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  1. F. A. Cumar
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  2. B. Maggio
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  3. R. Caputto
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Cumar, F.A., Maggio, B. & Caputto, R. Ganglioside-cholera toxin interactions: a binding and lipid monolayer study. Mol Cell Biochem 46, 155–160 (1982). https://doi.org/10.1007/BF00239664

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  • DOI: https://doi.org/10.1007/BF00239664

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