Skip to main content
SpringerLink
Log in

Platelet calcium pump activity in essential hypertensives and their first-degree relatives

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Intracellular free Ca2+ concentration has been shown to be elevated in platelets of patients with essential hypertension. This study was designed to characterize Ca2+-pump activity of the platelet membranes (surface and intracellular) in these patients. A double-blind study was carried out. Untreated and treated (on R-blockers) essential hypertensives were studied in comparison with normotensive control subjects. First degree blood relatives of essential hypertensives were also studied. The Ca2+-activation kinetics of the enzyme showed a significant decrease in the Vmax. (for the plasma- and intracellular membranes) and Km (for the intracellular membranes) in the essential hypertensive patients. Increased platelet membrane cholesterol content was observed in these patients. Lowered Ca2+-efflux by Ca2+-ATPase may lead to elevated intracellular free Ca2+-levels in platelets of essential hypertensives. A lowered Ca2+-ATPase activity may emerge as a marker for essential hypertension.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Shanbaky NM, Ahn Y, Jy W, Harrington W, Fernandez L, Hanes DH: Abnormal aggregation accompanies abnormal platelet Cal' handling in arterial thrombosis. Thromb. Haemost 57: 1–10, 1987

    Google Scholar 

  2. Bruschi G, Bruschi ME, Caroppo M, Orlandini G, Spaggiari M, Cavatorta A: Cytoplasmic free [Ca2+]i is increased in the platelets of spontaneously hypertensive rats and essential hypertensive patients. Clin Sci 68: 179–184, 1985

    Google Scholar 

  3. Taylor MA, Ayers CR, Gear ARL: Platelet calcium and quenched-flow aggregation kinetics in essential hypertension. Hypertension 13: 558–566, 1989

    Google Scholar 

  4. Gulati S, Khullar M, Sagar S, Ganguly NK, Sharma BK: Ca2+ transport in unstimulated platelets of essential hypertensives and their blood relatives. J Hypertens 10: 1213–1219, 1992

    Google Scholar 

  5. Ahn YS, Jy W, Harrington WJ, Shanbaky N, Fenandez LF, Haynes DH: Increased platelet calcium in thrombosis and related disorders and its correction by nifedipine. Thromb Res 45: 135–143, 1987

    Google Scholar 

  6. Resink TJ, Tkachuk VA, Erne P, Buhler FR: Platelet membrane calmodulin-stimulated calcium adenosine triphosphatase altered activity in essential hypertension. Hypertension 8: 159–166, 1986

    Google Scholar 

  7. Takaya J, Lasker N, Bamforth R, Gutkin M, Byrd LH, Aviv A: Kinetics of Ca2+-ATPase activation in platelet membranes of essential hypertensives and normotensives. Am J Physiol 258: C988-C994, 1990

    Google Scholar 

  8. Dean WL, Pope JE, Brier ME, Aronoff GR: Platelet calcium transport in hypertension. Hypertension 23: 31–37, 1994

    Google Scholar 

  9. Enouf J, Bredoux R, Bourdeau N, Sarkadi B, Levy-Toledano S. Further characterization of the plasma membrane and intracellular membrane-associated platelet Call transport systems. Biochem J 263: 547–552, 1989

    Google Scholar 

  10. Sixma JJ and Lips JPM: Isolation of platelet membranes. Thromb Haemost 39: 328–337, 1978

    Google Scholar 

  11. Aronson NN and Touster O. Biomembranes (Part A) In: S. Fleischer and L. Packer (eds). Methods in Enzymology, Academic Press, New York, Vol. 31, 1974, pp. 93–94

    Google Scholar 

  12. Chen PS, Toribara TY and Warner H: Microdetermination of phosphorus. Anal Chem 28: 1756–1758, 1956

    CAS  Google Scholar 

  13. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951

    CAS  PubMed  Google Scholar 

  14. Zlatkis A, Zak B and Boyle AJ: A new method for the direct determination of serum cholesterol. J Lab Clin Med 41: 486–1192, 1953

    Google Scholar 

  15. Astarie C, Levenson J, Simon A, Meyer P, Devynck M: Platelet cytosolic proton and free calcium concentrations in essential hypertension. J Hypertens 7: 485–491, 1989

    Google Scholar 

  16. Erne P, Bolli P, Burgisser E, Buhler FR: Correlation of platelet calcium with blood pressure: effect of antihypertensive therapy. N Engl J Med 310: 1084–1088, 1984

    Google Scholar 

  17. Shinitzky M: Membrane fluidity and cellular functions. In: M.B. Shinitzky (ed.). Physiology of Membrane fluidity, CRC Press, pp. 1–52, 1984

  18. Neyses L, Locher R, Stimpei M, Streuli R, Vetter W: Stereospecific modulation of the calcium channel in human erythrocytes by cholesterol and its oxidized derivatives. Biochem 227: 105–112, 1985

    Google Scholar 

  19. Locher R, Knorr M, Edmonds D, Neyses L, Vetter W: Influence of cholesterol on intracellular free calcium concentration in human platelets. J Hypertens 4 (Suppl. 6): 5358–5360, 1986

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India

    S. Gulati, M. Khullar & N. K. Ganguly

  2. Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India

    B. K. Sharma

Authors
  1. S. Gulati
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Khullar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. K. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. K. Ganguly
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gulati, S., Khullar, M., Sharma, B.K. et al. Platelet calcium pump activity in essential hypertensives and their first-degree relatives. Mol Cell Biochem 156, 37–42 (1996). https://doi.org/10.1007/BF00239317

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00239317

Key words

Search

Navigation