Summary
The lateral cotyledonary meristems present in germinating seed were inoculated with a non-oncogenic strain of A. tumefaciens carrying a gene conferring kanamycin resistance as a selectable marker and a β-glucuronidase sequence as a reporter gene. Kanamycin resistant plants were derived from the meristems and shown to be transformed on the basis of Southern blots, polymerase chain reaction analysis and tests for β-glucuronidase activity. The plants were fertile and tests of their progeny confirmed the transmission of integrated sequences through a sexual generation. This transformation method has the merit of an unlimited supply of material for inoculation and a relatively short time scale from inoculation to the production of rooted plants.
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Abbreviations
- BAP:
-
benzyl amino purine
- GA3:
-
gibberellic acid
- GUS:
-
β=glucuronidase
- IBA:
-
indole butyric acid
- MS:
-
Murashige and Skoog medium
- NAA:
-
naphthalene acetic acid
- NPTII:
-
neomycin phosphotransferase II
- PCR:
-
polymerase chain reaction
- TO:
-
first generation transformants
- T1:
-
second generation transformants
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Communicated by H. Lörz
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Davies, D.R., Hamilton, J. & Mullineaux, P. Transformation of peas. Plant Cell Reports 12, 180–183 (1993). https://doi.org/10.1007/BF00239102
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DOI: https://doi.org/10.1007/BF00239102