Summary
Smears of fresh rat brain tissue combined with immunohistochemistry using antiserum to glial fibrillary acidic protein (GFA) were used to visualize individual astrocytes in different cortical regions of rats ranging in age from 1 to 30 months. By computerized image analysis, the cell area and the cell perimeter were determined. Using 4-month-old male Sprague-Dawley rats, it was found that GFA-positive astrocytes from cerebellum and hippocampus were significantly larger, both in terms of cell area and cell perimeter, than similar cells from cortex cerebri. The temporal development was carefully followed in smears of the hippocampal formation where a continuous increase in cell size was observed from 1 to 30 months of age. During the first few postnatal months a rapid increase in both cell area and cell perimeter was observed using Sprague-Dawley rats. For studies of senescent animals, Fisher 344 rats specifically bred for aging studies were obtained. Using such animals, a second, highly significant slower growth phase which continued until the longest time points studied was observed. A separate experiment using Sprague-Dawley rats also showed large differences in both cell area and cell perimeter of GFA-positive cerebellum and cortical astrocytes taken from 6-week- and 18-month-old animals. In conclusion, the present study shows that maturation of GFA-positive astrocytes is a process which continues for several months postnatally. This relatively rapid growth phase is followed by a slower increase in cell size, probably continuing throughout life.
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References
Bignami A, Eng LF, Dahl D, Uyeda CT (1972) Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res 43: 429–435
Bignami A, Dahl D (1973) Differentiation of astrocytes in the cerebellar cortex and the pyramidal tracts of the newborn rat. An immunofluorescence study with antibodies to a protein specific to astrocytes. Brain Res 49: 393–402
Bignami A, Dahl D (1974a) Astrocyte specific protein and neuroglial differentiation. An immunofluorescence study with antibodies to glial fibrillary acidic protein. J Comp Neurol 153: 27–38
Bignami A, Dahl D (1974b) Astrocyte-specific protein and radial glia in the cerebral cortex of newborn rat. Nature (Lond) 252: 55–56
Bignami A, Dahl D, Rueger C (1980) Glial fibrillary acidic (GFA) protein in normal neural cells and in pathological conditions. In: Fedoroff S, Hertz L (eds) Advances in cellular neurobiology, Vol I. Academic Press, New York London, pp 285–319
Björklund H, Dahl D, Olson L (1984a) Morphometry of GFA and vimentin positive astrocytes in grafted and lesioned cortex cerebri. Int J Dev Neurosci 2: 181–192
Björklund H, Eriksdotter-Nilsson M, Dahl D, Olson L (1984b) Astrocytes in smears of CNS tissues as visualized by GFA and vimentin immunofluorescence. Med Biol 62: 38–48
Choi BH, Lapham LW (1980) Evolution of Bergmann glia in developing human fetal cerebellum: a Golgi, electron microscopic and immunofluorescence study. Brain Res 190: 369–383
Cicero IJ, Terrenedelli JA, Suntzeff V, Moore BW (1972) Regional changes in CNS levels of the S-100 and 14–3–2 proteins during development and aging in the mouse. J Neurochem 19: 2119–2125
Coons AH (1958) Fluorescent antibody methods. In: Danielli JF (ed) General cytochemical methods. Academic Press, New York, pp 399–422
Dahl D, Bignami A (1976) Immunogenic properties of the glial fibrillary acidic protein. Brain Res 116: 150–157
Dahl D, Rueger DC, Bignami A, Weber K, Osborn M (1981) Vimentin, the 57,000 dalton protein of fibroblast filaments, is the major cytoskeletal component in immature glia. Eur J Cell Biol 124: 191–196
Dahl D (1981) The vimentin-GFA protein transition in rat neuroglia cytoskeleton occurs at the time of myelination. J Neurosci Res 6: 741–748
Dalton MM, Hommes OR, Leblond CP (1968) Correlation of glial proliferation with age in the mouse brain. J Comp Neurol 134: 397–400
Eng LF, DeArmond SJ (1983) Immunochemistry of the glial fibrillary acidic protein. In: Zimmerman HM (ed) Progress in neuropathology, Vol 5. Raven Press, New York, pp 19–39
Feldman ML, Dowd C (1975) Loss of dendritic spines in aging cerebral cortex. Anatomy Embryology 148: 279–301
Ghandour MS, Labourdette G, Vincendon G, Gombos G (1981) A biochemical and immunohistological study of S100 protein in developing rat cerebellum. Dev Neurosci 4: 98–109
Goldman G, Coleman P (1981) Neuron numbers in locus coeruleus do not change with age in Fisher 344 rats. Neurobiol Aging 2: 33–36
Haglid KG, Hansson H-A, Rönnbäck L (1977) S-100 in the central nervous system of rat, rabbit and guinea pig during postnatal development. Brain Res 123: 331–345
Herschman HR, Levine L, DeVellis J (1971) Appearance of a brain-specific antigen (S100 protein) in the developing rat brain. J Neurochem 18: 629–633
Heumann D, Leuba G (1983) Neuronal death in development and aging of the cerebral cortex of the mouse. Neuropathol Appl Neurobiol 9: 297–312
Kaplan SM, Hinds JW (1980) Gliogenesis of astrocytes and oligodendrocytes in the neocortical grey and white matter of the adult rat: electron microscopic analysis of light radioautographs. J Comp Neurol 193: 711–727
Landfield PW, Rose G, Sandles L, Wohlstadter TC, Lynch G (1977) Patterns of astroglial hypertrophy and neuronal degeneration in the hippocampus of aged, memory-deficient rats. J Gerontol 32: 3–12
Latov N, Nilaver G, Zimmerman EA, Johnson WG, Silverman A-J, Defendini R, Cote L (1979) Fibrillary astrocytes proliferate in response to brain injury: a study combining immunoperoxidase technique for glial fibrillary acidic protein and radioautography of tritiated thymidine. Dev Biol 72: 381–384
Leuba G (1983) Aging of dendrites in the cerebral cortex of the mouse. Neuropathol Appl Neurobiol 9: 467–475
Levitt P, Rakic P (1980) Immunoperoxidase localization of glial fibrillary acidic protein in radial glial cells and astrocytes of the developing rhesus monkey brain. J Comp Neurol 193: 815–840
Ling EA, Leblond CP (1973) Investigation of glial cells in semithin sections. II. Variation with age in the numbers of the various glial cell types in rat cortex and corpus callosum. J Comp Neurol 149: 73–82
Ludwin SK, Kosek JC, Eng LF (1976) The topographical distribution of S-100 and GFA proteins in the adult rat brain: an immunohistochemical study using horseradish peroxidase-labelled antibodies. J Comp Neurol 165: 197–208
Luse S (1968) In: Winkler J (ed) Pathology of the nervous system, Vol 1. McGraw Hill, New York, pp 538–553
Masoro E (1980) Mortality and growth characteristics of rat strains commonly used in aging research. Exp Aging Res 6: 219–233
Murray HM, Walker BE (1973) Comparative study of astrocytes and mononuclear leukocytes reacting to brain trauma in mice. Exp Neurol 41: 290–302
O'Kusky J, Colonnier M (1982) Postnatal changes in the number of astrocytes, oligodendrocytes, and microglia in the visual cortex (area 17) of the macaque monkey: a stereological analysis in normal and monocularly deprived animals. J Comp Neurol 210: 307–315
Olson L, Ungerstedt U (1970) Monoamine fluorescence in CSN smears: sensitive and rapid visualization of nerve terminals without freeze-drying. Brain Res 17: 343–347
Paterson J, Privat A, Ling EA, Leblond CP (1973) Investigation of glial cells in semithin sections. III. Transformation of sub-ependymal cells into glial cells, as shown by radioautography after 3H-thymidine injection into the lateral ventricle of the brain of young rats. J Comp Neurol 149: 83–102
Raju T, Bignami A, Dahl D (1981) In vivo and in vitro differentiation of neurons and astrocytes in the rat embryo: immunofluorescence study with neurofilament and glial filament antisera. Dev Biol 85: 344–357
Takashima S, Becker LE (1983) Developmental changes of glial fibrillary acidic protein in cerebral white matter. Arch Neurol 40: 14–18
Vaughan DW, Peters A (1974) Neuroglial cells in the cerebral cortex of rats from young adulthood to old age: an electron-microscope study. J Neurocytol 3: 405–429
Vaughn JE, Peters A (1967) Electron microscopy of the early postnatal development of fibrous astrocytes. Am J Anat 121: 131–152
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Björklund, H., Eriksdotter-Nilsson, M., Dahl, D. et al. Image analysis of GFA-positive astrocytes from adolescence to senescence. Exp Brain Res 58, 163–170 (1985). https://doi.org/10.1007/BF00238964
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DOI: https://doi.org/10.1007/BF00238964