Summary
A comparative immunohistological study of the neurone-specific γγ enolase and αα enolase, demonstrates the exclusive neuronal localization of γγ enolase and its absence from glial cells. In contrast, αα enolase is located in astroglial cells. The validity of γγ enolase as a neuronal marker and αα enolase as an astrocytic marker, is confirmed both by a double labelling technique, using antibodies to αα and to γγ revealed with fluorescence or peroxidase in the same tissue sections, and by immunoelectronmicroscopy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baranowski T, Wolna E (1975) Enolase from human muscle. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 42. Academic Press, New York, pp 335–338
Bock E, Dissing J (1975) Demonstration of enolase activity connected to the brain specific protein 14.3.2. Scand J Immunol [Suppl 2] 4: 31–36
Bock E, Fletcher L, Rider CC, Taylor CB (1978) The nature of the two proteins of brain specific antigen 14.3.2. J Neurochem 30: 181–185
Brightman MW, Anders JJ, Schmechel D, Rosenstein JM (1978) The lability of the shape and content of glial cells. In: Schoffeniels E, Frank G, Tower DB, Hertz L (eds) Dynamic properties of glia cells. Pergamon Press, Oxford, pp 21–44
Eng LF, Bigbee JW (1978) Immunohistochemistry of nervous system-specific antigens. Adv Neurochem 3: 43–92
Fletcher L, Rider CC, Taylor CB (1976) Enolase isoenzymes. III. Chromatographic and immunological characteristics of rat brain enolase. Biochim Biophys Acta 452: 245–252
Fletcher L, Rider CC, Taylor CB, Adamson ED, Luke BM, Graham CF (1978) Enolase isoenzymes as markers of differentiation in teratocarcinoma cells and normal tissues of mouse. Dev Biol 65: 462–475
Ghandpur MS, Langley OK, Vincendon G, Gombos G (1979) Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers. J Histochem Cytochem 27: 1634–1637
Ghandour MS, Langley OK, Gombos G, Vincendon (1980a) Cellular localization of 14–3–2 protein and carbonic anhydrase in rat cerebellum in the optical and electron microscope. In: Balazs R, Di Benedetta C, Gombos G, Porcellati (eds) A multidisciplinary approach to brain development. Elsevier, Amsterdam, pp 35–36
Ghandour MS, Langley OK, Vincendon G, Gombos G, Filippi D, Limozin N, Dalmasso C, Laurent G (1980b) Immunochemical and immunohistochemical study of carbonic anhydrase II in adult rat cerebellum. A marker for oligodendrocytes. Neuroscience 5: 559–571
Hartmann BK, Cimino M, Moore BW, Agrawal HC (1977) Immunohistochemical localization of brain specific proteins during development. Trans Am Soc Neurochem 8: 66
Keller A, Margolis FL (1976) Isolation and characterization of rat olfactory marker protein. J Biol Chem 251: 6232–6237
Keller A, Scarna H, Legault L, Zeitoun Y, Lando D, Cousin M (1979) Comparative changes in isozymic forms of neuron specific enolase (NSE) during brain development and in vitro differentiation of nervous cell lines. 7th Meeting of ISN, Jerusalem 2–6 Sept. Abstr.
Langley OK, Ghandour MS, Vincendon G, Gombos G (1980) An ultrastructural immunocytochemical study of nerve specific protein in rat cerebellum. J Neurocytol (in press)
Marangos PJ, Zomzely-Neurath C, York C (1976) Determination and characterization of neuron specific protein (NSP) associated enolase activity. Biochem Biophys Res Commun 68: 1309–1316
Marangos PJ, Zis AP, Clark RL, Goodwin FK (1978) Neuronal, non neuronal and hybrid forms of enolase in brain. Structural, immunological, and functional comparisons. Brain Res 150: 117–133
Moore BW, McGregor D (1965) Chromatographic and electrophoretic fractionation of soluble proteins of brain and liver. J Biol Chem 240: 1647–1653
Pickel WM, Reis DJ, Marangos PJ, Zomzely-Neurath C (1976) Immunocytological localization of nervous system specific protein (NSP-R) in rat brain. Brain Res 105: 184–187
Rider CC, Taylor CB (1974) Enolase isoenzymes in rat tissues. Electrophoretic, immunological and kinetics properties. Biochem Biophys Acta 365: 285–300
Rider CC, Taylor CB (1975) Evidence for a new form of enolase in rat brain. Biochem Biophys Res Commun 66: 814–820
Schmechel DE, Marangos PJ, Ziz AP, Brightman MW, Goodwin FK (1978a) Brain enolases as specific markers of neuronal and glial cells. Science 199: 313–315
Schmechel DE, Marangos PJ, Brightman MW (1978b) Neuronspecific enolase is a marker for- central and peripheral neuroendocrine cells. Nature 276: 834–836
Zomzely-Neurath C, Keller A (1977) The different forms of brain enolase. Isolation, characterization, cell specificity and physiological significance. In: Roberts S, Lajtha A, Gispen WH (eds) Mechanisms, regulation and special function of protein synthesis in the brain. Elsevier, Amsterdam, pp 279–298
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ghandour, M.S., Langley, O.K. & Keller, A. A comparative immunohistological study of cerebellar enolases. Exp Brain Res 41, 271–279 (1981). https://doi.org/10.1007/BF00238884
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00238884