Skip to main content
SpringerLink
Log in

Water loss and polyethylene glycol-mediated acclimatization of in vitro-grown seedlings of 5 cultivars of date palm (Phoenix dactylifera L.) plantlets

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Plantlets derived from shoot-tips of seedlings from five cultivars of date palm, Phoenix dactylifera L., were subjected to polyethylene glycol in liquid medium. Comparisons of water loss of detached leaves among in vitro-grown, polyethylene glycol-treated and greenhouse-grown plants showed significant differences with treatment for all cultivars studied. For each treatment, significant differences were also found among cultivars. The common result was that the percent of moisture loss of non-treated in vitro-grown plantlets was almost twice that of greenhouse-grown plants. Polyethylene glycol-treated plantlets showed a water loss of approximately 27%, similar to that of greenhouse plants as compared to an average of 40% in control plants. This demonstrates the possibility of using polyethylene glycol as an osmoticum for in vitro acclimatization of plantlets prior to transfer to soil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EW:

epicuticular wax

MS:

Murashige and Skoog (1962) medium

NAA:

α naphthalene acetic acid

PEG:

polyethylene glycol

PPFD:

photosynthetic photon flux density

References

  • Bhaskaran S, Smith RH, Newton RJ (1985) Plant Physiol. 79:266–269

    Google Scholar 

  • Boxus P, Quoirin M (1977) Acta Horticulturae 78:373–378

    Google Scholar 

  • Brainerd KE, Fuchigami LH (1981) J. Amer. Soc. Hort. Sci. 106:515–518.

    Google Scholar 

  • Conner AJ, Thomas MB (1981) The Inter. Plant Prop. Soc. Combined Proceedings 31:342–357

    Google Scholar 

  • Conner LN, Thomas MB (1981) Inter. Plant Prop. Soc. Comb. Proc. 31:342–357

    Google Scholar 

  • Crane J, Hughes HG (1990) HortScience 25(7):794–795

    Google Scholar 

  • Dami I (1991) M.Sci. Thesis. Colorado State University, Fort Collins, CO, 115 pp

    Google Scholar 

  • Dami I, Hughes HG (1991) HortScience 26:725 (Abstr.)

    Google Scholar 

  • Debergh PC (1991) Acta Horticulturae 289-291-300

  • Donnelly DJ, Skelton FE, Nelles JE (1987) J. Amer. Soc. Hort.Sci. 112(3):566–569

    Google Scholar 

  • Grout BWW, Crisp P (1977) Acta Horticulturae 78:289–296

    Google Scholar 

  • Heyser JW, Nabors MW (1981) Plant Physiol. 68:1454–1459

    Google Scholar 

  • Kawasaki J, Akiba T, Moritsugu M (1983) Plant and Soil 75:75–85

    Google Scholar 

  • Lee NI, Wetzstein H, Sommer HE (1985) Plant Physiol. 78:637–641

    Google Scholar 

  • Mohammed GH, Vidaver WE (1990) Plant Cell, Tissue and Organ Culture 21:111–117

    Google Scholar 

  • Murashige T (1974) Ann. Rev. Plant Physiol. 25:135–166

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497

    Google Scholar 

  • Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff Publishers, Dodrecht, Netherlands

    Google Scholar 

  • Preece JE, Sutter EG (1991) In: P.C. Debergh and R.H. Zimmerman (eds.), Micropropagation: Technology and Applications. Kluwer Academic Publishers, Dordrecht, pp. 71–93

    Google Scholar 

  • SAS (1985) SAS User's Guide: Statistics, version 5 (ed.) SAS Institute, Inc. Cary, N.C.

    Google Scholar 

  • Safadi F (1992) Ph.D. Dissertation. Colorado State University, Fort Collins, CO

    Google Scholar 

  • Short KC, Warburton J, Roberts AV (1987) Acta Horticulturae 212:329–334

    Google Scholar 

  • Sutter EG (1988) J. Amer. Soc. Hort. Sci. 113(2):234–238

    Google Scholar 

  • Sutter EG, Langhans RW (1982) Can. J. Bot. 60:2896–2902

    Google Scholar 

  • Wardle K, Dobbs EB, Short KC (1983) J. Amer. Soc. Hort. Sci. 389

  • Zaid A (1990) Ph.d. Dissertation. Colorado Stete University, Fort Collins, CO (Diss. Abstr. 91 DAI 51/068), 147 pp

    Google Scholar 

  • Zaid A, Tisserat BH (1983) Date Palm Jour. 2(2):163–182

    Google Scholar 

  • Zaid A, Hughes HG (1989) HortScience 24:122 (Abstr.)

    Google Scholar 

  • Ziv M (1986) In: L.A. Withers and P.G. Alderson (eds.). Plant tissue culture and its agricultural applications. Butterworths, London, pp. 187–196

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, Semlalia, P.O. Box S15, Marrackech, Morocco

    Abdelouahhab Zaid

  2. Department of Horticulture, Colorado State University, 80523, Fort Collins, CO, USA

    Harrison Hughes

Authors
  1. Abdelouahhab Zaid
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Harrison Hughes
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Parrott

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zaid, A., Hughes, H. Water loss and polyethylene glycol-mediated acclimatization of in vitro-grown seedlings of 5 cultivars of date palm (Phoenix dactylifera L.) plantlets. Plant Cell Reports 14, 385–388 (1995). https://doi.org/10.1007/BF00238602

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00238602

Keywords

Search

Navigation