Summary
A cDNA encoding the potato (Solanum tuberosum L.) chloroplast ribosomal protein S16 (cp rps16) was isolated and characterized. The deduced amino acid sequence of the chloroplast rps16 coding sequence from potato shows 33% homology to the E.coli ribosomal protein S16 and 95% homology to the tobacco chloroplast ribosomal protein S16. The 13 amino acids encoded by exon-1 are highly conserved (100% identity) among higher plants. Two sizes of rps16 transcripts have been identified from northern blot analysis. The 1.5 kb transcript contains the 855 bp intron and represents an unprocessed RNA molecule, whereas the 0.7 kb transcript represents the mature form. The expression of rps16 is developmentally regulated, with transcript levels decreasing during tuber development and increasing during shoot development. The potato cp rps16 gene appears to be constitutively expressed in unswollen stolons and axillary buds, and its expression is enhanced by light.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- cp:
-
chloroplast
- rps :
-
ribosomal protein small subunit
References
Aguettaz P, Seyer P, Pesey H, Lescure A-M (1987) Plant Mol Biol 8:169–177
Baumgartner B, Rapp JC, Mullet JE (1993) Plant Physiol 101:781–791
Byström AS, Hjalmarsson KJ, Wikström PK, Björk GR (1983) EMBO J 2(6):899–905
Capel MS, Bourque DP (1982) J. Biol chem 257:7746–7755
Dabeva MD, Post-Beittenmiller MA, Warner JR (1986) Proc Natl Acad Sci USA 83:5854–5857
Ewing EE (1985) In: P. Li (ed.) Potato Physiology, Academic Press, Orlando, FL., USA, pp. 154–207
Hansen JD, Hannapel DJ (1992) Plant Physiol 100:164–169
Hannapel DJ (1991) Physiol Plant 83:568–573
Johnson R, Ryan CA (1990) Plant Mol Biol 14:527–536
Kanakari S, Timmler G, von Knoblauch K, Subramanian AR (1992) Plant Mol Biol 18:419–422
Kang S-G, Hannapel DJ (1994) Plant Physiol (in press)
Maid U, Zetsche K (1992) Plant Mol Biol 19:1001–1010
Marinos NG (1967) J Ultrastruct Res 17:91–113
Mignery GA, Pikarrd CS, Hannapel DJ, Park WD (1984) Nucleic Acids Res 12:7987–8000
Mullet JE (1988) Ann Rev Plant Physiol Plant Mol Biol 39:475–502
Neuhaus H, Scholz A, Link G (1989) Curr Genet 15:63–70
Nomura M, Course R, Baughman (1984) Annu Res Bioch 53:75–117
Presutti C, Ciafre S-A, Bozzoni I (1991) EMBO J 10:2215–2221
Sambrook J, Fritsch EF, Maniatis T (1989) In: Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA
Sexton TB, Jones JT, Mullet JE (1990) Curr Genet 17:445–454
Shinozaki K, Deno H, Sugita M, Kuramitsu S, Sugiura M (1986a) Mol Gen Genet 202:1–5
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986b) EMBO J 5 (9):2043–2049
Stern DB, Gruissem W (1987) Cell 51:1145–1157
Stern S, Changchien L-M, Craven GR, Noller HF (1988) J Mol Biol 200:291–299
Subramanian AR (1985) Essays Bjochem 21:15–85
Suh SG, Stiekema WJ, Hannapel DJ (1991) Planta 184:423–430
Tanaka M, Wakasugi T, Sugita M, Shinozaki K, Sugiura M (1986) Proc Natl Acad Sci USA 83:6030–6034
Thomson WW, Whatley JM (1980) Ann Rev Plant Physiol 31:375–394
Wenzler HC, Mignery GA, Fisher LM, Park WD (1989) Plant Mol Biol 12:41–50
Author information
Authors and Affiliations
Additional information
Communicated by C.F. Quiros
Rights and permissions
About this article
Cite this article
Kang, SG., Polking, G.F. & Hannapel, D.J. Expression of a cDNA for chloroplast ribosomal protein S16 of potato (Solanum tuberosum L.). Plant Cell Reports 14, 359–365 (1995). https://doi.org/10.1007/BF00238597
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00238597