Summary
The total uptake and incorporation into DNA of 3H-thymidine in MCF-7 cells is stimulated by estradiol after an incubation period of 24 h. Prolonged incubations show a decreased rate of both uptake and incorporation of 3H-thymidine, but the labeling index remains elevated up to 48 h.
It appears that this is the only exception to the observed good relationship between labeling index and total uptake of 3H-thymidine. This correlation may be of use in comparing autoradiographic and biochemical data on DNA synthesis in the case of culturing both primary mammary tumors and mammary cancer cell lines.
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References
Mitchison, J. M., 1973. The biology of the cell cycle, pp. 11–16; 197–200.
Soule, H. D., Vasquez, J., Long, A., Albert, S. and Brennan, M., 1973. J. Nat. Cancer Inst. 51: 1409–1416.
Lippman, M., Bolan, G. & Huff, K., 1976. Cancer Res. 36: 4595–4601.
Lippman, M., Bolan, G. & Huff, K., 1976. Cancer Res. 36: 4610–4618.
Lippman, M., Bolan, G. & Huff, K., 1976. Cancer Res. 36: 4602–4609.
Osborne, C. K., Monaco, M. E., Lippman, M. E. & Kahn, C. R., 1978. Cancer Res. 38: 94–102.
Burke, R. E. and McGuire, W. L., 1978. Cancer Res. 38: 3769–3773.
Shiu, R. P. C., 1979. Cancer Res. 39: 4381–4386.
Lowry, O. H., Rosebaugh, N. J., Farr, A. L. & Randall, R. J., 1951. J. Biol. Chem. 193: 265–275.
Lippman, M., Bolan, G., Monaco, M., Pinkus, L. & Engel, L., 1976. J. Steroid Biochem. 7: 1045–1051.
Horwitz, K. B., Koseki, Y. & McGuire, W. L., 1978. Endocrinology 103, 1742–1751.
Jozan, S., Moure, C., Gillois, M. & Bayard, F., 1979. J. Steroid Biochem. 10: 341–342.
Zava, D. T., Chamness, G. C., Horwitz, K. B. & McGuire, W. L., 1977. Nature 196: 663–664.
Rademaker, B., Vossenberg, J. B. J., Poortman, J. & Thijssen, J. H. H., 1980. J. Steroid Biochem. 13: 787–791.
Weichselbaum, R. R., Hellman, S., Piro, A. J. Nove, J. J. & Little, J. B., 1978, Cancer Res. 38: 2339–2342.
Horwitz, K. B., Zava, D. T., Thilager, A. K., Jensen, E. M. & McGuire, W. L., 1978. Cancer Res. 38: 2334–2437.
Bresciani, F., 1965. Exp. Cell Res. 38: 13–32.
Epifanova, O. I., 1966. Exp. Cell Res. 42: 562–577.
Das, R. M., 1972. J. Endocrinol. 55: 21–30.
Galand, P., Leroy, F. & Chrétien, J., 1971. J. Endocrinol. 49: 243–252.
Gerschenson, L. E., Conner, E. & Murai, J. T., 1977. Endocrinology 100: 1468–1471.
Davison, P., Liu, S. & Karasek, M., 1979. Cell Tissue Kinet. 12: 605–614.
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Coosen, R., de Jong, P.W.J. & Schwarz, F. Influence of estradiol on the total uptake and incorporation of thymidine in human breast cancer (MCF-7) in long-term cell culture. Mol Cell Biochem 42, 155–160 (1982). https://doi.org/10.1007/BF00238509
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DOI: https://doi.org/10.1007/BF00238509