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Restoration by cyclic AMP of the differentiated phenotype of chondrocytes from de-differentiated cells pretreated with retinoids

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Summary

Parathyroid hormone (PTH) increases the cyclic AMP level in rabbit costal chondrocytes in culture. PTH, dibutyryl cyclic AMP (DBcAMP), and 8-bromo cyclic AMP (8-Br cAMP) induce ornithine decarboxylase (ODC) and expression of the differentiated phenotype of chondrocytes in this cell system. On the other hand, retinoids inhibit expression of the differentiated phenotype of chondrocytes. In the present study, the effects of PTH, DBcAMP, and 8-Br cAMP on rabbit costal chondrocytes pretreated with retinoids were examined.

PTH did not increase the cellular cyclic AMP level in de-differentiated cells that had been pretreated with retinyl acetate or retinoic acid for three days, but it did increase the cyclic AMP level four days after removal of retinoids. PTH did not stimulate ODC activity or expression of the differentiated phenotype of chondrocytes in the de-differentiated state. On the other hand, DBcAMP or 8-Br cAMP stimulated expression of the differentiated phenotype of chondrocytes even in de-differentiated cells, as judged by morphological and bistological changes of the cells and increase in glycosaminoglycan synthesis. Cyclic AMP analogues also induced ODC in these cells.

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Author notes

  1. Masaharu Takigawa

    Present address: Dept. of Oncology, McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI, USA

Authors and Affiliations

  1. Dept. of Biochemistry and Calcified-Tissue Metabolism, Faculty of Dentistry, Osaka University, 3-48, Nakanoshima 4-Chome, 530, Kita-Ku, Osaka, Japan

    Masaharu Takigawa, Teruko Takano & Fujio Suzuki

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  1. Masaharu Takigawa
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  2. Teruko Takano
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  3. Fujio Suzuki
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Takigawa, M., Takano, T. & Suzuki, F. Restoration by cyclic AMP of the differentiated phenotype of chondrocytes from de-differentiated cells pretreated with retinoids. Mol Cell Biochem 42, 145–153 (1982). https://doi.org/10.1007/BF00238508

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  • DOI: https://doi.org/10.1007/BF00238508

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