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Effects of phytic acid on the myoglobin-t-butylhydroperoxide-catalysed oxidation of uric acid and peroxidation of erythrocyte membrane lipids

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Abstract

Phytic acid stimulated the myoglobin-t-butylhydroperoxide (TBHP)-catalysed oxidation of uric acid, but inhibited the peroxidation of erythrocyte membrane lipids induced by the same system. Butylated hydroxy-toluene, a free radical chain reaction-terminating antioxidant, also suppressed the myoglobin-TBHP-induced lipid peroxidation. Moreover, phytic acid inhibited the hydroxyl radical-induced degradation of deoxyribose, but the extent of inhibition in this system was reduced by increasing the ferric ion concentration, suggesting that these effects of phytic acid on the myoglobin-TBHP-mediated oxidation are more likely attributable to its metal chelating properties rather than to a free radical scavenging action. The effectiveness of phytic acid, a naturally occurring antioxidant, in the inhibition of both iron- (as previously shown) and myoglobin-dependent lipid peroxidation suggests its possible therapeutic application as a non-toxic antioxidant for ameliorating the extent of oxy-radical-mediated myocardial ischemia/reperfusion damage.

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Abbreviations

ASC:

Ascorbic acid

BHT:

Butylated Hydroxytoluene

DMSO:

Dimethyl Sulfoxide

TBHP:

t-Butylhydroperoxide

TBA:

Thiobarbituric Acid

TBARS:

Thiobarbituric Acid-reactive Substances

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Authors and Affiliations

  1. Department of Pharmacology & Therapeutics, Faculty of Medicine, The University of British Columbia, 2176 Health Sciences Mall, V6T 1W5, Vancouver, B. C., Canada

    Kam Ming Ko & David V. Godin

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  1. Kam Ming Ko
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  2. David V. Godin
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Ko, K.M., Godin, D.V. Effects of phytic acid on the myoglobin-t-butylhydroperoxide-catalysed oxidation of uric acid and peroxidation of erythrocyte membrane lipids. Mol Cell Biochem 101, 23–29 (1991). https://doi.org/10.1007/BF00238434

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  • DOI: https://doi.org/10.1007/BF00238434

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