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Calcium-binding protein in the developing rat cerebellum

An immunocytochemical study

Cell and Tissue Research volume 233, pages 389–402 (1983)Cite this article

Summary

Specific antibodies raised against a human 28 000 dalton cerebellar calcium-binding protein (CaBP) were used in an immunocytochemical study during development of the rat cerebellum. Both light and electron microscopy showed (1) that labelling was entirely restricted to the Purkinje cells, (2) that it appeared very early in Purkinje cell development, (3) that the entire cell was labelled from the tip of the smallest dendrites to the axonal terminals, and (4) that with increasing age, the immunoreaction appeared to be progressively restricted to the cell and organelle membranes.

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Author information

Affiliations

  1. Laboratoire de Physiologie comparée, ERA 955 du CNRS “Neurobiologie du Développement et Endocrinologie”, Université des Sciences et Techniques du Languedoc, Montpellier, France

    Ch. Legrand, M. Thomasset, C. O. Parkes, M. C. Clavel & A. Rabié

  2. Unité de Recherches sur le Métabolisme Hydro-minéral, INSERM U 120, Le Vésinet, France

    M. Thomasset

  3. Department of Physiology, University of British Columbia, Vancouver, Canada

    C. O. Parkes

Authors
  1. Ch. Legrand
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  2. M. Thomasset
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  3. C. O. Parkes
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  4. M. C. Clavel
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  5. A. Rabié
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Legrand, C., Thomasset, M., Parkes, C.O. et al. Calcium-binding protein in the developing rat cerebellum. Cell Tissue Res. 233, 389–402 (1983). https://doi.org/10.1007/BF00238305

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  • DOI: https://doi.org/10.1007/BF00238305

Key words

  • Calcium-binding protein (CaBP)
  • Immunocytochemistry
  • Rat cerebellum
  • Development
  • Vitamin D