Summary
Motoneurons of the sciatic nerve were studied in the lumbar cord of the mouse, at various postnatal stages, 1–3 days, 6–7 days, three weeks, and 2–3 months. Horseradish peroxidase (HRP) was applied at the cut end of the distal sciatic nerve, and, after retrograde transport, transversal and longitudinal histological sections of the cord were made in order to compare various features of the motor pool such as cell grouping, position in gray matter, cell number, cell size, and gross dendritic patterns. As early as birth, sciatic nerve motoneurons were grouped in a longitudinal column in the homolateral gray matter. No labelled cells were found outside the column either ipsilaterally or contralaterally. The location of the column was constant throughout postnatal development, encompassing the same spinal root levels and occupying the same position in the gray matter, the lateral portion of the ventral horn. Cell volume showed a 4–5 fold increase. Dendrites were not visible at the early stages and became labelled at 2–3 months. The neuropil also displayed a 4–5 fold increase, causing considerable spacing of the motoneurons. Histograms of cell body volume showed the progressive differentiation of the initially uniform motoneuronal pool in different sub-populations.
In each experimental animal, labelled cells were counted in all histological sections and, for statistical comparison, the value was corrected for mean cell size. A 31% decrease in the number of labelled cells was observed between birth and 2–3 months. The rate of cell death was not constant, since 17% disappeared during the first week and 14% thereafter. The 31% decrease was lower than that observed in previous studies in the rat, 50–80%. The difference in species may explain this discrepancy, however, technical differences must also be invoked. In these studies, HRP was injected into the biceps brachii muscle. An overestimation of cell death is possible with this technique because postnatal changes of the axonal endings permit easier HRP utpake in immature muscles than in mature muscles. The cut nerve technique however by-passes the axonal endings.
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References
Abercrombie M (1946) Estimation of nuclear population from microtome sections. Anat Rec 94: 239–247
Adams JC (1980) Stabilizing and rapid thionin staining of TMB-based HRP reaction product. Neurosci Lett 17: 7–9
Angulo AW (1940) The differentiation of the motor cell columns in the cervical cord of albino rat fetuses. J Comp Neurol 72: 469–488
Barker D (1974) The morphology of muscle receptors. In: Hunt CC (ed) Muscle receptors. Springer, Berlin Heidelberg New-York (Handbook of Sensory Physiology, vol 3, part 2, pp 1–190)
Baulac M, Meininger V (1980) Topographical arrangement of motoneurons from the ventral muscles of the arm in rat. Neurosci Lett [Suppl] 5: 87
Baulac M, Meininger V (1981) Organization of the motor neurons of pectoral musculature in the rat. Contribution to the study of the axillary arch (Achselbogen). Acta Anat (Basel) 109: 209–217
Brown MC, Jansen JKS, Van Essen O (1976) Polyneuronal innervation of skeletal muscle in new-born rats and its elimination during maturation. J Physiol (Lond) 261: 387–422
Burke RE, Strick PL, Kanda K, Kim CC, Walmsley B (1977) Anatomy of medial gastrocnemius and soleus motor nuclei in cat spinal cord. J Neurophysiol 40: 667–680
Chu-Wang IW, Oppenheim RW (1978) Cell death of motoneurons in the chick embryo spinal cord. I. A light and electron microscopic study of naturally occuring and induced cell loss during development. J Comp Neurol 177: 33–57
Cowan WM (1979) Selection and control in neurogenesis. In: Schmitt FO, Worden FG (eds) The Neurosciences: fourth study program. The MIT Press, Cambridge, MA, pp 59–79
Eccles JC, Sherrington CS (1930) Numbers and contraction values of individual motor units examined in some muscles of the limb. Proc R Soc Lond [Biol] 106: 326–357
Ford DH, Cohan G (1968) Changes in weight and volume of rat spinal cord motor neurons with increasing age. Acta Anat (Basel) 71: 311–319
Goering JH (1928) An experimental analysis of the motor cells columns in the cervical enlargement of the spinal cord in the albino rat. J Comp Neurol 46: 125–151
Hadley RT, Trachtenberg MC (1978) Poly-L-Ornithine enhances the uptake of horseradish peroxidase. Brain Res 158: 1–14
Hamburger V (1975) Cell death in the development of the lateral motor column of the chick embryo. J Comp Neurol 160: 535–546
Harris-Flanagan AE (1969) Differentiation and degeneration in the motor horn of the foetal mouse. J. Morphol 129: 281–306
Henneman E, Somjen G, Carpenter DO (1965) Functional significance of cell size in spinal motoneurons. J Neurophysiol 28: 560–580
Hughes A (1961) Cell degeneration in the larval ventral horn of Xenopus Laevis. J Embryol Exp Morphol 9: 269–284
Hughes A (1973) The development of dorsal root ganglia and ventral horns in opossum. A quantitative study. J Embryol Exp Morphol 30: 359–376
Jansen JKS, Thompson W, Kuffler DP (1978) The formation and maintenance of synaptic connections as illustrated by studies of the neuromuscular junction. In: Corner MA, Baker RE, van de Pol NE, Swaab DR, Uylings HBM (eds) Maturation of the nervous system. Elsevier, Amsterdam (Progress in brain research, vol 48, pp 3–19)
Kennedy WR, Yoon KS (1979) Permeability of muscle spindle capillaries and capsule. Muscle Nerve 2: 101–108
Konigsmark BW (1970) Methods for counting neurons. In: Nauta WJH, Ebbesson SOE (eds) Contemporary research methods in neuroanatomy. Springer, Berlin Heidelberg New York, pp 315–340
Kristensson K, Olsson Y (1971) Retrograde axonal transport of protein. Brain Res 29: 363–365
Lamb AH (1977) Neuronal death in the development of the somatotopic projections of the ventral horn in xenopus. Brain Res 134: 145–150
Landon DN (1972) The fine structure of developing muscle spindles in the rat. J Physiol (Lond) 11: 512–513
Lavelle A, Lavelle FW (1959) Neuronal reaction to injury during development: severance of the facial nerve in utero. Exp Neurol 1: 82–95
Livett BG (1976) Axonal transport and neuronal dynamics: contributions to the study of neuronal connectivity. In: Porter R (ed) Neurophysiology II. University Park Press, Baltimore (International review of physiology, vol 10, pp 37–124)
Marinesco G (1904) Les localisations médullaires chez le chien et chez l'homme. Sem Med (Paris) 29: 225–231
Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26: 106–117
Mesulam MM, Brushart TM (1979) Transganglionic and anterograde transport of horseradish peroxidase across dorsal root ganglia: a tetramethylbenzidine method for tracing central sensory connections of muscles and peripheral nerves. Neuroscience 4: 1107–1117
Prestige MC (1967) The control of cell number in the lumbar ventral horns during the development of xenopus laevus tadpoles. J Embryol Exp Morphol 18: 359–387
Reed AF (1940) The nuclear masses in the cervical spinal cord of Macaca Mulatta. J Comp Neurol 72: 187–206
Richmond FJR, Scott DA, Abrahams VC (1978) Distribution of motoneurons to the neck muscles, biventer cervicis, splenius and complexus in the cat. J Comp Neurol 181: 451–464
Romanes GJ (1946) Motor localization and the effects of nerve injury on the ventral horn cells of the spinal cord. J Anat 80: 117–131
Romanes GJ (1951) The motor-cell columns of the lumbo-sacral cord in the cat. J Comp Neurol 94: 313–363
Rootman DS, Tatton WB, Hay M (1981) Post-natal histogenetic death of rat forelimb motoneurons. J Comp Neurol 199: 17–27
Sakla FB (1959) Post-natal growth of the cervical spinal cord of the albino mouse and the dendritic organization of its ventral horn cells. J Comp Neurol 113: 491–505
Sato M, Mizuno N, Konishi A (1977) Post-natal differentiation of cell body volumes of spinal motoneurons innervating slowtwitch and fast-twich muscles. J Comp Neurol 175: 27–36
Schade JP, Van Harreveld A (1961) Volume disitribution of moto- and interneurons in the peroneus-tibialis neurons pool of the cat. J Comp Neurol 117: 387–398
Scheibel ME, Scheibel AB (1966) Spinal motoneurons, interneurons and Renshaw cells. A Golgi study. Arch Ital Biol 104: 328–353
Scheibel ME, Scheibel AB (1970) Developmental relationship between spinal motoneuron dendrites bundle and patterned activity in the hindlimb of cats. Exp Neurol 29: 328–335
Sprague JM (1948) A study of motor cell localization in the spinal cord of the rhesus monkey. Am J Anat 82: 1–26
Sterling P, Kuypers HGHM (1967) Anatomical organization of the brachial spinal cord of the cat. II. The motoneuron plexus. Brain Res 4: 16–32
Strick PL, Burke RE, Kanda K, Kim CC, Walmsley B (1976) Differences between alpha and gamma motoneurons labelled with horseradish peroxidase by retrograde transport. Brain Res 113: 582–588
Tada K, Ohshita S, Yonenobu K, Ono K, Satoh K, Shimizu N (1979) Development of spinal motoneuron innervation of the upper limb muscle in the rat. Exp Brain Res 35: 287–293
Tweedle CD, Stephens KE (1981) Development of complexity in motor nerve endings at the rat neuromuscular junction. Neuroscience 6: 1657–1662
Zacks SI, Saito A (1969) Uptake of exogeneous horseradish peroxidase by coated vesicles in mouse neuromuscular junctions. J Histochem Cytochem 17: 161–170
Zelena J (1964) Development, degeneration and regeneration of receptor organs. In: Singer M, Schade JP (eds) Mechanisms of neural regeneration. Elsevier, Amsterdam (Progress in Brain Research, vol 13, pp 175–213)
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Baulac, M., Meininger, V. Postnatal development and cell death in the sciatic motor nucleus of the mouse. Exp Brain Res 50, 107–116 (1983). https://doi.org/10.1007/BF00238237
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DOI: https://doi.org/10.1007/BF00238237