Summary
Unusual paracrystalline aggregates of microtubules which have not been described in any other mammalian species were observed in cultured anterior pituitary cells of normal chinchillas as well as in situ in the pituitary glands of these animals. These aggregates appeared as regularly arranged tubular structures in the longitudinal plane, and as a checkerboard pattern of closely and regularly packed microtubules when examined in transverse section. Supplementation with vinblastine, colcemide or colchicine in the culture medium did not change these structures morphologically. Each unit of tubules consisted of an outer wall or parellelogram profile and an inner wall composed of a single hexagonal doublet or in a figure “8” form. The outer wall of the parallelogram was 35×28 nm in length for both sides, while the diagonal of the inner wall was 18×28 nm. These paracrystalline aggregates of microtubules in the chinchilla pituitary cells are morphologically distinct from the paracrystalline assembly of cytoplasmic microtubules induced by vinblastine or other alkaloids.
The function and significance of these paracrystalline aggregates in anterior pituitary cells of the chinchilla are uncertain.
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References
Bensch KG, Malawista SE (1969) Microtubular crystals in mammalian cells. J Cell Biol 40:95–107
Hirano A, Zimmerman HM (1970) Some effects of vinblastine implantation in the cerebral white matter. Lab Invest 23:358–367
Labrie F, Gauthier M, Pelletier G, Borgeat P, Lemay A, Gouge JJ (1973) Role of microtubules in basal and stimulated release of growth hormone and prolactin in rat adenohypophysis in vitro. Endocrinology 93:903–914
Lacy PE, Malaisse WJ (1973) Microtubules and beta cell secretion. Recent Prog Horm Res 29:199–228
MacLeod RM, Lehmeyer JE, Bruni E (1973) Effect of antimitotic drugs on the in vitro secretory activity of mammotrophs and somatrophs and on their microtubules. Proc Soc Exp Biol Med 144:259–267
Nicoll CS, Meites J (1962) Estrogen stimulation of prolactin production by rat adenohypophysis in vitro. Endocrinology 70:272–277
Shiino M (1979) Morphological changes of pituitary gonadotrophs and thyrotrophs following treatment with LH-RH or TRH in vitro. Cell Tissue Res 202:399–406
Shiino M, Rennels EG (1974) Paracrystalline aggregates of microtubules in the anterior pituitary cells of the chinchilla (Chinchilla laniger). Am J Anat 139:135–140
Shiino M, Rennels EG (1975) Vinblastine-induced microtubular paracrystals in prolactin cells of anterior pituitary gland of lactating rats. Am J Anat 144:399–405
Shiino M, Rennels EG (1976) Electron microscopic observations on depression of pituitary prolactin secretion by vinblastine in the rat. Gunma Symp Endocrinol 13:195–206
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Tashjian AH, Barowsky NJ, Jensen DK (1971) Thyrotrophin releasing hormone: Direct evidence for stimulation of prolactin production by pituitary cells in culture. Biochem Biophys Res Comm 43:516–523
Tyson GE, Bulger RE (1973) Vinblastine-induced paracrystals and unusually large microtubules (macrotubules) in rat renal cells. Z Zellforsch 141:443–458
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Supported by USPHS Grant HD 11826
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Shiino, M. Ultrastructural observation of paracrystalline aggregates of microtubules in anterior pituitary cells of the chinchilla (Chinchilla laniger). Cell Tissue Res. 213, 433–440 (1980). https://doi.org/10.1007/BF00237889
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DOI: https://doi.org/10.1007/BF00237889