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Cholinergic transmission in subcortical and cortical visual centers of rats: No evidence for the involvement of primary optic system

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Summary

The effect of unilateral enucleation, ablation of the visual cortex or coagulation of the lateral geniculate nucleus (LGN) upon the activity of choline acetyltransferase (ChAc) and acetylcholinesterase (AChE) in different structures of the visual system of albino rats was studied. The localization and extent of the degeneration pattern were followed up by histological silver degeneration methods. Afferents from the retina project mainly contralaterally to the dorsal and ventral LGN, the pretectal region and the superior colliculus. Afferent fibres from the dorsal LGN enter the visual cortex in area 17 only. Neurons of this area project back ipsilaterally to the LGN and the superior colliculus (SC).

No significant decrease in the activity of the cholinergic marker enzyme choline acetyltransferase could be observed under any of the experimental conditions; there was rather a tendency to increased activity in the subcortical centres. AChE as a less specific marker also exhibited no gross changes in activity in the lesioned animals. The results add more direct proof to pharmacological and physiological evidence that ACh is not involved in the synaptic transmission of the direct optic projections in rats, either at the subcortical or at the cortical level.

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References

  • Bigl, V.: Modified microassay for the rapid and sensitive determination of choline acetyltransferase activity using (3H)-acetyl CoA and Fonnum's extraction procedure. Acta biol. med. germ. 34, 1437–1440 (1975)

    Google Scholar 

  • Bigl, V., Schober, W.: Transmitter systems and fiber connections in the visual system of rats. In: Abstracts of papers presented at the symposium “Differentiation of neuron and glia and their metabolic and functional relationships”. Castle Liblice, Czechoslovakia 1974

  • Bigl, V., Biesold, D., Weisz, K.: The influence of functional alteration on monoamine oxidase and catechol-O-methyl transferase in the visual pathway of rats. J. Neurochem. 22, 505–509 (1974)

    Google Scholar 

  • Bowsher, D.: Reticular projections to lateral geniculate in cat. Brain Res. 23, 247–249 (1970)

    Google Scholar 

  • Chatfield, P.O., Cord, J.T.: Effects of atropine, prostigmine and acetylcholine on evoked cortical potentials. Electroenceph. clin. Neurophysiol. 7, 533–556 (1955)

    Google Scholar 

  • Collier, B., Mitchell, J.F.: The central release of acetyl-choline during stimulation of the visual pathway. J. Physiol. (Lond.) 184, 239–254 (1966)

    Google Scholar 

  • Collier, B., Mitchell, J.F.: The central release of acetyl-choline during consciouness and after brain lesions. J. Physiol. (Lond.) 188, 83–98 (1967)

    Google Scholar 

  • Cobbin, L.B., Leeder, S., Pollard, J.: Smooth muscle stimulants in extracts of optic nerves, optic tracts and lateral geniculate bodies of sheep. Brit. J. Pharmacol. 25, 295–306 (1965)

    Google Scholar 

  • Curtis, D.R., Davis, R.: The excitation of lateral geniculate neurons by quarternary ammonium derivates. J. Physiol. (Lond.) 165, 62–82 (1963)

    Google Scholar 

  • David, J.P., Murayama, S., Machne, X., Unna, K.R.: Evidence supporting cholinergic transmission at the lateral geniculate body of the cat. Int. J. Neuropharmacol. 2, 113–125 (1963)

    Google Scholar 

  • Deffenu, G., Bertaccini, G., Pepeu, G.: Acetylcholine and 5-hydroxytryptamine levels of the lateral geniculate bodies and superior colliculus of cats after visual deafferentiation. Exp. Neurol. 17, 203–209 (1967)

    Google Scholar 

  • Domino, E.F.: Evidence for a functional role of acetylcholine in the lateral geniculate nucleus. In: The James E. P. Toman Memorial Volume. Advances in Biochemical Psychopharmacology (ed. H. Sabelli), pp. 95–107. New York: Raven Press 1973

    Google Scholar 

  • Domino, E.F., Krause, R.R., Bowers, J.: Regional distribution of some enzymes involved with putative neurotransmitters in the human visual system. Brain Res. 58, 179–189 (1973)

    Google Scholar 

  • Ebel, A., Massarelli, R., Sensenbrenner, M., Mandell, P.: Choline acetyltransferase and acetylcholinesterase activities in chicken brain hemispheres in vivo and in cell culture. Brain Res. 76, 461–472 (1974)

    Google Scholar 

  • Feldberg, W., Vogt, M.: Acetylcholine synthesis in different regions of the central nervous system. J. Physiol. (Lond.) 107, 372–381 (1948)

    Google Scholar 

  • Fink, R.P., Heimer, L.: Two methods for selective silver impregnation of degenerating axons and their synaptic endings in the central nervous system. Brain Res. 4, 369–374 (1967)

    Google Scholar 

  • Fivková, E., Maršala, J.: Stereotaxic atlases for the cat, rabbit and rat. In: Bures, J., Petran, M., and Zachar, J.: Electrophysiological methods in biological research. Prague 1967

  • Fonnum, F.: Radiochemical micro-assay for the determination of choline acetyltransferase and acetylcholinesterase activities. Biochem. J. 115, 465–472 (1969)

    Google Scholar 

  • Fonnum, F.: Recent developments in biochemical investigations of cholinergic transmission. Brain Res. 62, 497–507 (1973)

    Google Scholar 

  • Gwynn, D.G., Flumerfelt, B.A.: Acetylcholinesterase in non-cholinergic neurons: A histochemical study of dorsal root ganglion cells in the rat. Brain Res. 34, 193–198 (1971)

    Google Scholar 

  • Hebb, C.O.: Choline acetylase in mammalian and avian sensory systems. Quart. J. exp. Physiol. 40, 176–186 (1955)

    Google Scholar 

  • Hebb, C.O.: CNS at the cellular level: identity of transmitter agents. Ann. Rev. Physiol. 32, 165–192 (1970)

    Google Scholar 

  • Hebb, C.O., Morris, D.: Identification of acetylcholine and its metabolism in nervous tissue. In: The structure and function of nervous tissue (ed. G.H. Borne), Vol. III, pp. 25–60. New York and London: Academic Press 1969

    Google Scholar 

  • Hebb, C.O., Silver, A.: Choline acetylase in the central nervous system of man and some other mammals. J. Physiol. (Lond.) 134, 718–728 (1956)

    Google Scholar 

  • Krnjević, K., Phillis, J.W.: Pharmacological properties of acetylcholine — sensitive cells in the cerebral cortex. J. Physiol. (Lond.) 166, 328–350 (1963)

    Google Scholar 

  • Lebbin, C., Waser, P.G.: Studies about the specifity of the histochemical localization of choline acetyltransferase. Histochemistry 45, 309–317 (1975)

    Google Scholar 

  • Leger, L., Sakai, K., Salvert, D., Touret, M., Jouvet, M.: Delineation of dorsal lateral geniculate afferents from the cat brain stem as visualized by the horseradish peroxidase technique. Brain Res. 93, 490–496 (1975)

    Google Scholar 

  • Lüth, H.-J., Schober, W., Winkelmann, E., Luppa, H.: Morphologisch-histochemische Untersuchungen über die Verbindungen der mesencephalen retikulären Formation (Area cuneiformis) zu subkortikalen Strukturen des visuellen Systems. Acta histochem. (Jena) 57, 114–126 (1976)

    Google Scholar 

  • Matsuoka, J., Domino, E.F.: Cholinergic modulation of single lateral geniculate neurons in the cat. Neuropharmacology 11, 241–251 (1972)

    Google Scholar 

  • Miller, E., Heller, A., Moore, R.Y.: Acetylcholine in rabbit visual system nuclei after enucleation and visual cortex ablation. J. Pharmacol. exp. Ther. 165, 117–125 (1969)

    Google Scholar 

  • McLennan, H.: Synaptic transmission. 2nd ed. Philadelphia-London-Toronto: W. B. Saunders Company 1970

    Google Scholar 

  • Nauta, W.H.J., Gygax, P.A.: Silver impregnation of degenerating axons in the central nervous system: A modified technique. Stain Technol. 29, 91–93 (1954)

    Google Scholar 

  • Neumann, E., Nachmansohn, D.: Nerve excitability — Toward an integrating concept. In: Biomembranes (ed. H. Eisenberg, E. Katchalski-Katzur, L.A. Mansen), Vol. 7, pp. 99–165. New York: Plenum Publ. Corp. 1974

    Google Scholar 

  • Palkama, A., Sipponen, J.: Light and electron histochemical observations on cholinesterase-containing centrifugal fibers in the optic nerve of the rat. Ann. Med. exp. Fenn. 46, 564–573 (1968)

    Google Scholar 

  • Phillis, J.W., Tebēcis, A.K., York, D.H.: A study of cholineceptive cells in the lateral geniculate nucleus. J. Physiol. (Lond.) 192, 695–713 (1967)

    Google Scholar 

  • Potempska, A., Gradkowska, M., Oderfeld-Nowak, B.: Early changes in acetylcholine pools in the hippocampus of the rat brain after septal lesions. J. Neurochem. 24, 787–789 (1975)

    Google Scholar 

  • Powers, M., Clark, G.: An evaluation of cresyl echt violett as a Nissl stain. Stain Technol. 30, 83–92 (1955)

    Google Scholar 

  • Reale, E., Luciano, L., Spitznas, M.: The fine structural localization of acetylcholinesterase activity in the retina and optic nerve of rabbits. J. Histochem. Cytochem. 19, 85–96 (1971)

    Google Scholar 

  • Schober, W.: Die primäre optische Projektion bei Albinoratten und pigmentierten Ratten. Anat. Anz. 137, 257–286 (1975)

    Google Scholar 

  • Schober, W., Winkelmann, E.: Der visuelle Cortex der Ratte. Cytoarchitektonik und stereotaktische Parameter. Z. Mikr.-anat. Forsch. 89, 431–446 (1975)

    Google Scholar 

  • Schober, W., Winkelmann, E.: Die geniculo-corticale Projection bei Albinoratten. J. Hirnforsch. 17, 561–580 (1976)

    Google Scholar 

  • Shute, C.C.D., Lewis, P.R.: The ascending cholinergic reticular system: neocortical, olfactory and subcortical projections. Brain 90, 497–520 (1967)

    Google Scholar 

  • Silver, A.: The Biology of Cholinesterases. North Holland Publishing Company, Amsterdam, Oxford, New York 1974

    Google Scholar 

  • Spehlmann, R., Daniels, J.C., Smathers, C.C.: Acetylcholine and the synaptic transmission of specific impulses to the visual cortex. Brain 94, 125–138 (1971)

    Google Scholar 

  • Tebēcis, A.K.: Studies on the identity of the optic nerve transmitter. Brain Res. 63, 31–42 (1973)

    Google Scholar 

  • Volkmann, R. von: Vergleichende Cytoarchitektonik der Regio occipitalis kleiner Nager und ihrer Beziehung zur Sehleistung. Z. Anat. Entwickl.-Gesch. 85, 561–657 (1928)

    Google Scholar 

  • Werner, L., Schober, W., Winkelmann, E., Mühlig, P., Kühne, Chr.: Konventionelle und automatische quantitative Untersuchung histologischer Präparate nach experimenteller Beeinflussung des visuellen Systems. Paper presented at the 71st Congress Internat. Anatomical Soc., Rostock-Warnemünde (GDR) 1976

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Authors and Affiliations

  1. Paul-FIechsig-Institute of Brain Research, Departments of Neurochemistry and Neuroanatomy, Karl-Marx-University, DDR-7039, Leipzig, Germany

    V. Bigl & W. Schober

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  1. V. Bigl
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  2. W. Schober
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Sponsored by a grant of the Ministry of Science and Technology of the GDR

The able technical collaboration of Mrs. Ursula Köhler, Mrs. Brigitte Sawatzke and Mrs. Hildegard Gruschka is greatly acknowledged.

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Bigl, V., Schober, W. Cholinergic transmission in subcortical and cortical visual centers of rats: No evidence for the involvement of primary optic system. Exp Brain Res 27, 211–219 (1977). https://doi.org/10.1007/BF00237699

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