Summary
Microprojectile bombardment was used to introduce the GUS reporter gene into sugarcane axillary meristems. Chimeric expression of this gene was observed in 20–40% of shoots regenerated from sugarcane meristems one month after particle bombardment. The linear pattern of GUS expression observed is consistent with periclinal division from single transformed meristematic cells. Meristems have advantages over callus cells as targets for microprojectile transformation, and have potential for introducing agronomically important genes into current commercial sugarcane varieties.
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Communicated by J. K. Vasil
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Gambley, R.L., Ford, R. & Smith, G.R. Microprojectile transformation of sugarcane meristems and regeneration of shoots expressing β-Glucuronidase. Plant Cell Reports 12, 343–346 (1993). https://doi.org/10.1007/BF00237432
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DOI: https://doi.org/10.1007/BF00237432