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Improvement of anther culture methods for doubled haploid production in barley breeding

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There is potential to accelerate cultivar development with a doubled haploid system for breeding line production. Anther culture methodology was evaluated for U.S.A. spring barley (Hordeum vulgare L.) breeding applications. Gelrite was found to be an acceptable replacement for ficoll in the induction medium to reduce costs while maintaining embryoid and plant production levels. Beneficial effects of 28 d cold pretreatment of donor spikes for anther culture were confirmed with Pacific Northwest USA barley genotypes. A 3 d mannitol solution pretreatment of fresh anthers was shown to be less effective for green plant production compared to 28 d cold pretreatment of donor spikes. Extended donor spike cold pretreatment from 28 to 42 d did not reduce anther culture productivity. Based on this research, anther culture techniques show promise for economical and convenient application in spring barley breeding.

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  • Flehinghaus T, Deimling S, Geiger HH (1991) Plant Cell Rep 10:397–400

    Google Scholar 

  • Hou L, Ullrich SE (1990) Barley Newsletter 33:115 (Abstr)

    Google Scholar 

  • Huang B, Sunderland N (1982) Ann Bot 49:77–88

    Google Scholar 

  • Hunter CP (1985) Plant Cell Rep 4:267–268

    Google Scholar 

  • Hunter CP (1988) PhD Thesis, Wye College, University of London

  • Kao KN (1981) Z Pfl Physiol 103:437–443

    Google Scholar 

  • Kao KN, Saleem M, Abrams S, Pedras M, Horn D, Mallard C (1991) Plant Cell Rep 9:595–601

    Google Scholar 

  • Kasha KJ, Cho UA, Ziauddin A (1992) In: Munck L (ed.) Barley Genet VI vol II. Proc Sixth Int Barley Genet Symp, July 22–27, 1991 Helsingborg, Sweden, Munksgaard Int Publ Ltd, Copenhagen pp. 793–806

    Google Scholar 

  • Kuhlmann U, Foroughi-Wehr B, (1989) Plant Cell Rep 8:71–81

    Google Scholar 

  • Luckett DJ, Smithard RA (1992) Aust J Agric Res 43:67–78

    Google Scholar 

  • Lyne RL, Bennett RI, Hunter CP (1986) In: Withers LA, Alderson PG (eds) Plant Tissue Culture and Its Agricultural Application. Univ of Nottingham. Butterworth, Guildford pp. 405–411

    Google Scholar 

  • Olsen FL (1987) Carlsberg Res Commun 52:393–404

    Google Scholar 

  • Pickering RA, Devaux P (1992) In: Sheury PR (ed) Barley Genetics, Molecular Biology and Biotechnology. C.A.B. Intl, Wallingford, UK pp. 519–547

    Google Scholar 

  • Powell W (1988) Plant Cell, Tissue and Organ Culture 12:291–297

    Google Scholar 

  • SAS (1988) SAS Institute Inc., Cary, NC, USA

  • Sorvari S (1986) Ann Agric Fenn 25:249–254

    Google Scholar 

  • Sunderland N, Huang B, Hills GJ (1984) J Exp Bot 85:521–530

    Google Scholar 

  • Szarejko I, Kasha KJ (1991) Cereal Res Comm 19:219–237

    Google Scholar 

  • Zhou H (1990) MS thesis Washington State Univ, Pullman

    Google Scholar 

  • Zhou H, Zheng Y, Konzak CF (1991) Plant Cell Rep 10:63–66

    Google Scholar 

Download references

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Communicated by G. C. Phillips

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Hou, L., Ullrich, S.E., Kleinhofs, A. et al. Improvement of anther culture methods for doubled haploid production in barley breeding. Plant Cell Reports 12, 334–338 (1993).

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