Summary
Four types of synapses: axo-axonic, axo-dendritic, axon-spine, and axo-somatic were distinguished in the supraoptic nucleus in the rat. The density of synaptic terminals (boutons) in this nucleus is 35.17·106/mm3 hence there are over 5 million boutons on each side, and on the average 596 per neuron. Only about one third of the axon terminals in this nucleus originate outside the nucleus and its immediate neighbourhood. Two thirds appear to be of intranuclear or otherwise of local origin. This could be explained by assuming either numerous intranuclear axon collaterals or interneurons having richly arborizing axons, or possibly both.
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References
Adams, J.H., Daniel, P.M., Prichard, M.M.L.: Degeneration and regeneration of hypothalamic nerve fibers in the neurohypophysis after pituitary stalk section in the ferret. J. comp. Neurol. 135, 121–144 (1969)
Bandanarayake, R.C.: Morphology of the accessory neurosecretory nuclei and of the retrochiasmatic part of the supraoptic nucleus of the rat. Acta anat. (Basel) 80, 14–22 (1971)
Bara, D., Bartók, I., Csapó, Zs.: Beiträge zur Ultrastruktur der hypothalamischen Neurosecretion. Endokrinologie 53, 385–396 (1968)
Barker, J.L., Crayton, J.W., Nicoll, R.A.: Antidromic and orthodromic responses of paraventricular and supraoptic neurosecretory cells. Brain Res. 33, 353–366 (1971)
Bock, R.: Über die Darstellbarkeit neurosecretorischer Substanz mit Chromalaun-Gallocyanin im Supraopticohypophysären System beim Hund. Histochemie 6, 382–369 (1966)
Bodian, D.: Studies on the diencephalon of the Virginia opossum. I. The nuclear pattern in the adult. J. comp. Neurol. 71, 259–323 (1939)
Bodian, D., Maren, T.H.: The effect of neuro- and adenohypophysectomy on retrograde degeneration in hypothalamic nuclei of the rat. J. comp. Neurol. 94, 485–512 (1951)
Dellmann, H.-D., Owsley, P.A.: Investigations on the hypothalamo-neurohypophysial neurosecretory system of the grass frog (Rana pipiens) after transection of the proximal neurohypophysis. I. Light microscopic findings in animals kept at 18 °C environmental temperature. Z. Zellforsch. 87, 1–16 (1968)
Diepen, R.: Der Hypothalamus. In: W. v. Möllendorf, Eds. Handbuch der mikroskopischen Anatomie des Menschen. Berlin-Göttingen-Heidelberg: Springer 1962
Dreifuss, J.J., Kelly, J.S.: Recurrent inhibition of antidromically identified rat supraoptic neurones. J. Physiol. (Lond.) 220, 87–103 (1972)
Eneström, S.: Nucleus supraopticus; a morphological and experimental study in the rat. Acta path. microbiol. scand. 185, 1–99 (1967)
Ferreya Moyano, H., de Olmos, J.S.: Studies on vesiculated hypothalamic neurosecretory cells in the dog. Experientia (Basel) 28, 578–579 (1972)
Floderus, S.: Untersuchungen über den Bau der menschlichen Hypophyse mit besonderer Berücksichtigung der quantitativen mikromorphologischen Verhältnisse. Acta path. microbiol. scand. Suppl. 53, 276 (1944)
Folenius, E.: Etude comparative de la citologie fine du noyau preoptique (NPO) et du noyau lateral (NLT) chez la Truite (Salmo irideus Gibb.) et chez la Perche (Perca fluviatilis L.). Comparison des deux types de neurosecretion. Gen. comp. Endoer. 3, 66–85 (1962)
Greving, R.: Beiträge zur Anatomie des Zwischenhirns und seiner Funktion. I. Der anatomische Aufbau der Zwischenhirnbasis und des anschließenden Mittelhirngebietes des Menschen. Z. Anat. Entwickl.-Gesch. 75, 579–620 (1925)
Güldner, F.-H., Wolff, I.R.: Dendro-dendritic synapses in the suprachiasmatic nucleus of the rat hypothalamus. J. Neurocytol. 3, 245–250 (1974)
Halász, B., Pupp, L.: Hormone secretion of the anterior pituitary gland after physical interruption of all nervous pathways to the hypophysiotropic area. Endocrinology 77, 553–562 (1965)
Jasinski, A.: Hypothalamic neurosecretion in the rat, Myotis myotis Borkhausen, during the period of hibernation and activity. In: W. Bargmann and B. Scharrer (Eds.), Aspects of Neuroendocrinology, pp. 301–309. Berlin-Heidelberg-New York: Springer 1970
Klein, M.J., Porte, A., Stutinsky, F.: Contacts synaptiques axo-axoniques dens las noyaux supraoptiques et paraventriculaires du rat et de la souris. C. R. Acad. Sci. (Paris) 267, 1007–1009 (1968)
Klüver, H., Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Neuropath. exp. Neurol. 12, 400–403 (1953)
Koizumi, K., Yamashita, H.: Orthodromic, antidromic excitation and interactions between neuroendocrine cells of the hypothalamus. Physiologist 12, 71 (1969)
Koizumi, K., Yamashita, H.: Studies of antidromically identified neurosecretory cells of the hypothalamus by intracellular and extracellular recordings. J. Physiol. (Lond.) 221, 683–705 (1972)
Magoun, H.W., Ranson, S.W.: Retrograde degeneration of the supraoptic nuclei after section of the infundibular stalk in the monkey. Anat. Rec. 75, 107–122 (1939)
Merrick, R.L.: A quantitative study of the supraoptic nucleus of rat, dog, and man. Anat. Rec. 88, 446–447 (1944)
Moll, J.: Regeneration of the supraoptico-hypophyseal and paraventriculo-hypophyseal tracts in the hypophysectomized rat. Z. Zellforsch. 46, 686–709 (1957)
Morton, A.: A quantitative analysis of the normal neuron population of the hypothalamic magnocellular nuclei in man and of their projections to the neurophypophysis. J. comp. Neurol. 136, 143–158 (1969)
Morton, A.: The time course of retrograde neuron loss in the hypothalamic magnocellular nuclei of man. Brain 93, 329–336 (1970)
Nicoll, R.A., Barker, J.L.: The pharmacology of recurrent inhibition in the supraoptic neurosecretory system. Brain Res. 35, 501–511 (1971)
O'Connor, W.J.: Atrophy of the supraoptic and paraventricular nuclei after interruption of the pituitary stalk in dogs. Quart. J. exp. Physiol. 34, 29–42 (1947)
Palay, S.L.: The fine structure of secretory neurons in the preoptic nucleus of the goldfish. Anat. Rec. 138, 417–443 (1960)
Palkovits, M., Csapó, S.T.: Mikroprojektions-Meßtisch für die Vereinfachung von Kernvariationsuntersuchungen. Z. mikr.-anat. Forsch. 67, 339–342 (1961)
Palkovits, M., Fischer, J.: Karyometric Investigations, 347 pp. Akadémiai Kiadó, Budapest, 1968
Palkovits, M., Magyar, P., Szentágothai, J.: Quantitative histological analysis of cerebellum in cat. I. Number and arrangement in space of the Purkinje cells. Brain Res. 32, 1–13 (1971)
Palkovits, M., Záborszky, L., Ambach, G.: Accessory neurosecretory cell groups in the rat hypothalamus. Acta morph. Acad. Sci. hung. 22, 21–33 (1974)
Peterson, R.P.: Synapses in the rat supraoptic nucleus. Anat. Rec. 151, 399 (1965)
Polenov, A.L., Senchik, J.I.: Synapses on neurosecretory cells of the supraoptic nucleus in white mice. Nature (Lond.) 221, 1423–1424 (1966)
Priymak, E.Kh., Hajós, F.: Synapses on the supraoptic neurosecretory neurons of the rat: an electron microscopic study. Acta morph. Acad. Sci. hung. 18, 55–62 (1970)
Raisman, G.: An ultrastructural study of the effects of hypophysectomy on the supraoptic nucleus of the rat. J. comp. Neurol. 147, 181–208 (1973)
Rasmussen, A.T.: Effect of hypophysectomy and hypophysial stalk resection on the hypothalamic nuclei of animals and man. Res. Publ. Ass. nerv. ment. Dis. 20, 245–269 (1940)
Rechardt, L.: Electron microscopic and histochemical observations on the supraoptic nucleus of normal and dehydrated rats. Acta physiol. scand. 77, Suppl. 329, 1–79 (1969)
Streefkerk, J.G.: Functional changes in the morphological appearance of the hypothalamohypophyseal neurosecretory and catecholaminergic neural system, and in the adenohypophysis of the rat. Amsterdam: Thesis 1967
Vincent, J.D., Hayward, J.N.: Activity of single cells in the osmoreceptor-supraoptic nuclear complex in the hypothalamus of the waking rhesus monkey. Brain Res. 23, 105–108 (1970)
Yamashita, H., Koizumi, K.: Excitation and inhibition of mammalian neurosecretory neurons. Physiologist 13, 351 (1970)
Yamashita, H., Koizumi, K.: Study of neurosecretory neurons in supraoptic nuclei of dogs by intracellular and extracellular recording. Proc. Int. Union Physiol. Sci. 9, 612 (1971)
Zambrano, D.: The effect of nialamide and L-Dopa on the synaptic endings of the neurosecretory neurons of the supraoptic nucleus of the rat. Neuroendocrinology 3, 99–106 (1968)
Zambrano, D., De Robertis, E.: The secretory cycle of supraoptic neurons in the rat. Z. Zellforsch. 73, 414–431 (1966)
Záborszky, L., Léránth, Cs., Makara, G.B., Palkovits, M.: Quantitative studies on the supraoptic nucleus in the rat. II. Fiber connections. Exp. Brain Res. (In press)
Záborszky, L., Palkovits, M., Léránth, Cs.: Some quantitative aspects of experimental degeneration methods in the rat hippocampus. (In preparation)
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Léránth, C., Záborszky, L., Marton, J. et al. Quantitative studies on the supraoptic nucleus in the rat. I. Synaptic organization. Exp Brain Res 22, 509–523 (1975). https://doi.org/10.1007/BF00237351
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DOI: https://doi.org/10.1007/BF00237351