Summary
Endogenous peroxidase-like activity was investigated with a combined light and electron microscopical technique in 15 cats. The lateral cervical nucleus, the dorsal column nuclei, and segments C6 and L5 of the spinal cord were incubated with diaminobenzidine-tetrahydrochloride (DAB) or tetramethylbenzidine (TMB). After histochemical reaction with DAB a considerable amount of activity was found in nerve cells, astrocytes and pericytes. The neuronal labelling was mainly located in mitochondria of axon terminals and in dendrites whereas the astrocytic and pericytic activity was found in cytoplasmic dense bodies. The quantity of stained structures differed considerably between the animals. In TMB reacted tissue endogenous peroxidase-like activity was only sparsely seen. It was found mainly in frozen sections, in which the neuropil and perivascular structures sometimes contained granules and irregular filaments. The significance of the findings is discussed in relation to observations in tracer studies using horseradish peroxidase.
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References
Broadwell RD, Brightman MW (1976) Entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood. J Comp Neurol 166: 257–284
Cancilla PA, Baker RN, Pollock PS, Frommes BS (1972) The reaction of pericytes of the central nervous system to exogenous protein. Lab Invest 26: 376–383
Carson KA, Mesulam M-M (1982) Electron microscopic tracing of neural connections with horseradish peroxidase. In: Mesulam M-M (ed) Tracing neural connections with horseradish peroxidase. John Wiley & Sons Ltd., New York, p 153
Conradi NG (1981) Endogenous peroxidatic activity in the cerebral and cerebellar cortex of normal adult rats. Acta Neuropathol 7: 3–6
Dietrichs E, Walberg F (1979) The cerebellar corticonuclear and nucleocortical projections in the cat as studied with anterograde and retrograde transport of horseradish peroxidase. 1.The paramedian lobe. Anat Embryol 158: 13–39
Divac I, LaVail JH, Rakic P, Winston KR (1977) Heterogeneous afferents to the inferior parietal lobule of the Rhesus monkey revealed by the retrograde transport method. Brain Res 123: 197–207
Holländer H (1970) The section embedding (SE) technique. A new method for the combined light microscopic and electron microscopic examination of central nervous tissue. Brain Res 20: 39–47
Keefer DA, Christ JF (1976) Distribution of endogenous diaminobenzidine-staining cells in the normal rat brain. Brain Res 116: 312–316
Koritsánszky S, Vigh B, Aros B (1967) Studies on Gomori-positive glial cells. 1. The changes in the periventricular Gomoripositive glial cells in rats of various ages. Acta Biol Hung 18: 9
Kumamoto T (1981) Histochemical study on endogenous diaminobenzidine-positive granules in the glial cell of rat brain. Acta Histochem Cytochem 14: 173–185
Land PW, Lund RD (1979) Development of the rat's uncrossed retinotectal pathway and its relation to plasticity studies. Science 205: 698–700
LaVail JH, LaVail MM (1974) The retrograde intraaxonal transport of horseradish peroxidase in the chick visual system: a light and electron microscopic study. J Comp Neurol 157: 303–358
Malmgren L, Olsson Y (1978) A sensitive method for histochemical demonstration of horseradish peroxidase in neurons following retrograde axonal transport. Brain Res 148: 279–294
McKenna O, Arnold G, Holtzman E (1976) Microperoxisome distribution in the central nervous system of the rat. Brain Res 117: 181–194
Mensah P, Finger T (1975) Neuromelanin: a source of possible error in HRP material. Brain Res 98: 183–188
Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26: 106–117
Olsson Y, Hossman KA (1970) Fine structural localization of exuded protein tracers in the brain. Acta Neuropath (Berl) 16: 103–116
Repérant J (1975) The orthograde transport of horseradish peroxidase in the visual system. Brain Res 85: 307–312
Rexed B (1952) The cytoarchitectonic organization of the spinal cord in the cat. J Comp Neurol 96: 415–495
Rosene DL, Mesulam M-M (1978) Fixation variables in horseradish peroxidase neurohistochemistry. I. The effects of fixation time and perfusion procedures upon enzyme activity. J Histochem Cytochem 26: 28–39
Sakumoto T, Nagai T, Kimura H, Maeda T (1980) Electron microscopic visualization of tetramethyl benzidine reaction product on horseradish peroxidase neurohistochemistry. Cell Mol Biol 26: 211–216
Schönitzer K, Holländer H (1981) Anterograde tracing of horseradish peroxidase (HRP) with the electron microscope using the tetramethylbenzidine reaction. J Neurosci Meth 4: 373–383
Sherlock DA, Field PM, Raisman G (1975) Retrograde transport of horseradish peroxidase in the magnocellular neurosecretory system of the rat. Brain Res 88: 403–414
Streit P, Reubi JC (1977) A new and sensitive staining method for axonally transported horseradish peroxidase (HRP) in the pigeon visual system. Brain Res 126: 530–537
Stürmer C, Bielenberg K, Spatz WB (1981) Electron microscopical identification of 3,3′,5,5′-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport. Neurosci Lett 23: 1–5
Vassar PS, Hards JM, Brooks DE, Hagenberger B, Seaman GVF (1972) Physicochemical effects of aldehydes on the human erythrocyte. J Cell Biol 53: 809–818
Walberg F, Brodal A, Hoddevik GH (1976) A note on the method of retrograde transport of horseradish peroxidase as a tool in studies of afferent cerebellar connections, particularly those from the inferior olive; with comments on the orthograde transport in Purkinje cell axons. Exp Brain Res 24: 383–401
Wong-Riley MTT (1976) Endogenous peroxidatic activity in brain stem neurons as demonstrated by their staining with diaminobenzidine in normal squirrel monkeys. Brain Res 108: 257–277
Wong-Riley MTT, Merzenich MM, Leake PA (1978) Changes in endogenous enzymatic reactivity to DAB induced by neuronal inactivity. Brain Res 141: 185–192
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Svensson, B.A., Rastad, J. & Westman, J. Endogenous peroxidase-like activity in the feline dorsal column nuclei and spinal cord. Exp Brain Res 55, 325–332 (1984). https://doi.org/10.1007/BF00237283
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DOI: https://doi.org/10.1007/BF00237283