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Behavioural effects mediated by unilateral nigral dopamine receptor stimulation in the rat

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Summary

Unilateral intranigral injections of dopamine in conscious rats pretreated with nialamide resulted in either ipsiversive or contraversive rotation depending upon the site of injection. Injection of dopamine (50 μg) into the zona compacta of the substantia nigra induced weak ipsiversive or mixed ipsiversive and contraversive rotation. Injection of dopamine (12.5–50.0 μg) into zona reticulata of substantia nigra induced only contraversive circling. Destruction of the ipsilateral medial forebrain bundle (MFB) using 6-hydroxydopamine (6-OHDA) abolished ipsiversive circling but enhanced contraversive circling produced by dopamine or apomorphine. The combination of a unilateral 6-OHDA lesion of MFB with a kainic acid or electrolesion of the ipsilateral strio-nigral and pallido-nigral pathways reduced contraversive circling to intranigral apomorphine (10 μg). Ipsiversive circling produced following intranigral injection of dopamine is dependent upon the integrity of ascending dopamine neurones. Contraversive rotation is independent of ascending dopamine pathways but is reliant upon afferent input to the substantia nigra from the striatum and/or globus pallidus.

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References

  • Aghajanian GK, Bunney BS (1977) Dopamine “autoreceptors”: pharmacological characterization by microiontophoretic single cell recording studies. Naunyn-Schmiedeberg's Arch Pharmacol 297: 1–7

    Google Scholar 

  • Andrews CD, Woodruff GN (1982) Turning behaviour following nigral injections of dopamine agonists and glycine. Eur J Pharmacol 84: 169–175

    Google Scholar 

  • Arnt J, Scheel-Kruger J (1979) GABA-ergic and glycinergic mechanisms within the substantia nigra: pharmacological specificity of dopamine-independent contralateral turning behaviour and interactions with other neurotransmitiers. Psychopharmacol 62: 267–277

    Google Scholar 

  • Beckstead RM, Domesick VB, Nauta WJH (1979) Efferent connections of the substantia nigra and ventral tegmental area in the rat. Brain Res 175: 191–217

    Google Scholar 

  • Björklund A, Lindvall O (1975) Dopamine in dendrites of substantia nigra neurones: suggestions for a role in dendritic terminals. Brain Res 83: 531–537

    Google Scholar 

  • Bunney BS, Aghajanian GK (1973) Electrophysiological effects of amphetamine on dopaminergic neurones. In: Snyder SH, Usdin E (eds) Frontiers in catacholamine research, pp 957–962

  • Bunney BS, Aghajanian GK (1978) d-amphetamine-induced depression of central dopamine neurones: evidence for mediation by both autoreceptors and a striato-nigral feedback pathway. Naunyn-Schmiedeberg's Arch Pharmacol 304: 255–261

    Google Scholar 

  • Carter CJ (1982) Topographical distribution of possible glutamatergic pathways from the frontal cortex to the striatum and substantia nigra in rats. Neuropharmacol 21: 379–383

    Google Scholar 

  • Costall B, Naylor RJ, Pinder RM (1974) Design of agents for stimulation of neostriatal dopaminergic mechanisms. J Pharm Pharmacol 26: 753–762

    Google Scholar 

  • De Groot J (1959) The rat forebrain in stereotaxic coordinates. Verh K Ned Akad Wet 52: 11–40

    Google Scholar 

  • Faull RLM, Carman JB (1968) Ascending projections of the substantia nigra in the rat. J Comp Neurol 132: 73–92

    Google Scholar 

  • Faull RLM, Mehler WR (1978) The cells of origin of nigrotectal, nigrothalamic and nigrostriatal projections in the rat. Neurosci 3: 989–1002

    Google Scholar 

  • Gale K, Guidotti A, Costa E (1977) Dopamine-sensitive adenylate cyclase: location in substantia nigra. Science 195: 503–505

    Google Scholar 

  • Geffen LB, Jessell TM, Cuello AC, Iversen LL (1976) Release of dopamine from dendrites in rat substantia nigra. Nature 260: 258–260

    Google Scholar 

  • Glick SD, Crane LA (1978) Function of nigral dopamine receptors? Brain Res 156: 380–381

    Google Scholar 

  • Grofova I, Ottersen OP, Rinvik E (1978) Mesencephalic and diencephalic afferents to the superior colliculus and periaqueductal gray substance demonstrated by retrograde axonal transport of horseradish peroxidase in the cat. Brain Res 146: 205–220

    Google Scholar 

  • Groves PM, Wilson CJ, Young SJ, Rebec GV (1975) Selfinhibition by dopaminergic neurones. Science 190: 522–529

    Google Scholar 

  • Groves PM, Young SJ, Wilson CJ (1976) Self-inhibition by dopaminergic neurones: disruption by (±)-α-methyl-ptyrosine pretreatment or anterior diencephalic lesions. Neuropharmacol 15: 755–762

    Google Scholar 

  • Hall MD, Jenner P, Kelly E, Marsden CD (1983) Differential anatomical location of 3H-N,n-propylnorapomorphine and 3H-spiperone binding sites in the striatum and substantia nigra of the rat. Br J Pharmacol 79: 599–610

    Google Scholar 

  • Herkenham M (1979) The afferent and efferent connections of the ventromedial thalamic nucleus in the rat. J Comp Neurol 183: 487–518

    Google Scholar 

  • Hopkins DA, Niessen LW (1976) Substantia nigra projections to the reticular formation, superior colliculus and central gray in the rat, cat and monkey. Neurosci Lett 2: 253–259

    Google Scholar 

  • Jones EG, Leavitt RY (1974) Retrograde axonal transport and the demonstration of non-specific projections to the cerebral cortex and striatum from thalamic intralaminar nuclei in the rat, cat and monkey. J Comp Neurol 154: 349–378

    Google Scholar 

  • Joyce JN, Davis RE, van Hartesveldt C (1981) Behavioural effects of unilateral dopamine injection into dorsal or ventralf striatum. Eur J Pharmacol 72: 1–10

    Google Scholar 

  • Kelly PH, Moore KE (1978) Dopamine concentrations in the rat brain following injections into the substantia nigra of baclofen, γ-aminobutyric acid, γ-hydroxybutyric acid, apomorphine and amphetamine. Neuropharmacol 17: 169–174

    Google Scholar 

  • Kilpatrick IC, Starr MS (1981) Involvement of dopamine in circling responses to muscimol depends on intranigral site of injection. Eur J Pharmacol 69: 407–419

    Google Scholar 

  • Konig JFR, Klippel RA (1963) The rat brain: a stereotaxic atlas of the forebrain and lower parts of the brain stem. Williams and Wilkins, Baltimore

    Google Scholar 

  • Kozlowski MR, Sawyer S, Marshall JF (1980) Behavioural effects and supersensitivity following nigral dopamine receptor stimulation. Nature 287: 52–54

    Google Scholar 

  • Leviel V, Cheramy A, Glowinski J (1979) Role of the dendritic release of dopamine in the reciprocal control of the two nigrostriatal dopaminergic pathways. Nature 280: 236–239

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193: 265–275

    CAS  PubMed  Google Scholar 

  • Maggi A, Bruno F, Cattabeni F, Groppetti A, Parenti M, Racagni G (1978) Apomorphine-induced inhibition of striatal dopamine release: role of dopaminergic receptors in substantia nigra. Brain Res 145: 180–184

    Google Scholar 

  • Murrin C, Gale K, Kuhar M (1979) Autoradiographic localisation of neuroleptic and dopamine receptors in the caudate-putamen and substantia nigra: effects of lesions. Eur J Pharmacol 60: 229–235

    Google Scholar 

  • Nieoullon A, Cheramy A, Glowinski J (1977) Interdependence of the nigrostriatal dopaminergic systems on the two sides of the brain in the cat. Science 198: 416–418

    CAS  PubMed  Google Scholar 

  • Olpe HR, Schellenberg H, Koella WP (1977) Rotational behaviour induced in rats by intranigral application of GABA-related drugs and GABA antagonists. Eur J Pharmacol 45: 291–294

    Google Scholar 

  • Quik M, Emson PC, Joyce E (1979) Dissociation between the presynaptic dopamine-sensitive adenylate cyclase and 3H-spiperone binding sites in rat substantia nigra. Brain Res 167: 355–365

    Google Scholar 

  • Reavill C, Jenner P, Leigh N, Marsden CD (1978) Turning behaviour induced by injection of muscimol or picrotoxin into the substantia nigra demonstrates dual GABA components. Neurosci Lett 12: 323–328

    Google Scholar 

  • Reisine TD, Nagy JI, Fibiger HC, Yamamura HI (1979) Localization of dopamine receptors in rat brain. Brain Res 169: 209–214

    Google Scholar 

  • Reubi JC, Iversen LL, Jessell TM (1977) Dopamine selectively increases 3H-GABA release from slices of rat substantia nigra in vitro. Nature 268: 652–654

    Google Scholar 

  • Reubi JC, Emson PC, Jessell TM, Iversen LL (1978) Effects of GABA, dopamine and substance P on the release of newly synthesized 3H-5-hydroxytryptamine from rat substantia nigra in vitro. Naunyn-Schmiedeberg's Arch Pharmacol 304: 271–275

    Google Scholar 

  • Ruffieux A, Schultz W (1980) Dopaminergic activation of reticulata neurones in the substantia nigra. Nature 285: 240–241

    Google Scholar 

  • Scheel-Kruger J, Arnt J, Magelund G (1977) Behavioural stimulation induced by muscimol and other GABA agonists injected into the substantia nigra. Neurosci Lett 4: 351–365

    Google Scholar 

  • Spano PF, Trabucchi M, Di Chiara G (1977) Localization of nigral dopamine-sensitive adenylate cyclase on neurons originating from the corpus striatum. Science 196: 1343–1345

    Google Scholar 

  • Starr MS, Summerhayes M (1982) Multifocal brain sites for apomorphine-induced circling and other stereotyped motor behaviour in the 6-hydroxydopamine-lesioned rat. Neurosci Lett 34: 277–282

    Google Scholar 

  • Van der Heyden JAM, Venema K, Korf J (1980) Biphasic and opposite effects of dopamine and apomorphine on endogenous GABA release in the rat substantia nigra. J Neurochem 34: 119–125

    Google Scholar 

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Authors and Affiliations

  1. University Department of Neurology, Institute of Psychiatry, and The Rayne Institute, King's College Hospital Medical School, SE5, Denmark Hill, London, England

    E. Kelly, P. Jenner & C. D. Marsden

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  1. E. Kelly
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  2. P. Jenner
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  3. C. D. Marsden
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Kelly, E., Jenner, P. & Marsden, C.D. Behavioural effects mediated by unilateral nigral dopamine receptor stimulation in the rat. Exp Brain Res 55, 243–252 (1984). https://doi.org/10.1007/BF00237275

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  • DOI: https://doi.org/10.1007/BF00237275

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