Skip to main content
SpringerLink
Log in

Morphological and functional study of the effect of isoproterenol on salivary gland cells

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The cellular mechanisms responsible for the structural and functional alterations produced in salivary glands by isoproterenol (ISP) were investigated. Postnatal rats were injected with ISP, and alterations in the structure and protein components of the submandibular glands were determined. Normal age-dependent protein patterns detected by electrophoresis are attributed to the biochemical and functional differentiation of proacinar and acinar secretory cells and correlate with structural changes in these cells. Structural changes induced by ISP involve the acinar cells without detectably altering terminal tubule cells. In addition, synthesis of a specific protein by the glands is enhanced by ISP treatment. By comparing protein patterns in the submandibular glands of control and treated rats, it was concluded that ISP increases synthesis of a specific salivary protein normally produced by proacinar cells during a limited period of glandular development. These results suggest that a neurally mediated regulatory mechanism that becomes altered by ISP plays a role in normal salivary gland development.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ball, W.D., Nelson, N.J.: A stage-restricted secretory system in the submandibular gland of the neonatal rat. Differentiation 10, 147–158 (1978)

    Google Scholar 

  • Bressler, R.S.: Fine structure of the differentiating acini in submandibular glands of isoproterenol treated rats. Am. J. Anat. 138, 431–448 (1973)

    Google Scholar 

  • Clark, Jr., S.L.: The thymus in mice of strain 129/J studied with the electron microscope. Am. J. Anat. 112, 1–33 (1963)

    Google Scholar 

  • Cutler, L.S., Chaudhry, A.P.: Cytodifferentiation of acinar cells of the rat submandibular gland. Dev. Biol. 41, 31–41 (1974)

    Google Scholar 

  • Davis, B.: Disc electrophoresis. II. Methods and applications to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427 (1964)

    Google Scholar 

  • Denny, P.C., Cope, P.: Age specific differentiations during the development of the rat submandibular gland. Differentiation 8, 105–111 (1977)

    Google Scholar 

  • Ekfors, T., Chang, W.W.L., Bressler, R.S., Barka, T.: Isoproterenol accelerates the postnatal differentiation of rat submandibular gland. Dev. Biol. 29, 38–47 (1972)

    Google Scholar 

  • Feiglin, B., Reade, P.C.: Protein patterns in normal and induced pathological salivary glands of mice. Aust. J. Exp. Biol. Med. Sci. 52, 173–184 (1974)

    Google Scholar 

  • Fernadez-Sorenson, A., Carlson, D.M.: Isolation of a “proline rich” protein from rat parotid glands following isoproterenol treatment. Biochem. Biophys. Res. Commun. 60, 249–256 (1974)

    Google Scholar 

  • Fukushima, M., Barka, T.: The effects of 5-bromodeoxyuridine and isoproterenol on the postnatal differentiation of rat submandibular gland. Am. J. Anat. 147, 159–182 (1976)

    Google Scholar 

  • Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)

    CAS  PubMed  Google Scholar 

  • Menaker, L., Cobb, C.M., Sheetz, J.H., Navia, J.M.: A new protein in the saliva from isoproterenol treated rats. Fed. Proc. 33, 696 (1974a)

    Google Scholar 

  • Menaker, L., Sheetz, J.H., Cobb, C.M., Navia, J.M.: Gel electrophoresis of whole saliva and associated histologic changes in submandibular glands of isoproterenol treated rats. Lab. Invest. 30, 341–349 (1974b)

    Google Scholar 

  • Radley, J.M.: Ultrastructural changes in the rat submaxillary gland following isoprenaline. Z. Zellforsch. 97, 196–211 (1969)

    Google Scholar 

  • Robinovitch, M.R., Keller, P.J., Johnson, D.A., Iversen, J.M., Kauffman, D.L.: Changes in rat parotid salivary proteins induced by chronic isoproterenol administration. J. Dent. Res. 56, 290–303 (1977)

    Google Scholar 

  • Schneyer, C.A.: Regulation of salivary gland size. In: Regulation of organ and tissue growth (R.J. Goss, ed.), pp. 211–232. New York: Academic Press 1972

    Google Scholar 

  • Schneyer, C.A., Hall, H.D.: Autonomic regulation of postnatal changes in cell number and size of rat parotid. Am. J. Physiol. 219, 1268–1272 (1970)

    Google Scholar 

  • Schneyer, C.A., Hall, H.D.: Effects of removal of superior cervical ganglion or auriculotemporal nerve on course of postnatal change in rat parotid amylase. Proc. Soc. Exp. Biol. Med. 140, 911–915 (1972)

    Google Scholar 

  • Schneyer, C.A., Shackleford, J.M.: Accelerated development of salivary glands of early postnatal rats following isoprotereneol. Proc. Soc. Exp. Biol. Med. 112, 320–324 (1963)

    Google Scholar 

  • Selye, H., Veilleux, R., Cantin, M.: Excessive stimulation of salivary gland growth by isoproterenol. Science 133, 44–15 (1961)

    Google Scholar 

  • Simson, J.A.V., Spicer, S.S., Hall, B.J.: Morphology and cytochemistry of rat salivary gland acinar secretory granules and their alterations by isoproterenol. J. Ultrastruct. Res. 48, 465–482 (1974)

    Google Scholar 

  • Simson, J.A.V., Dom, R.M., Sannes, P.L., Spicer, S.S.: Morphology and cytochemistry of acinar secretory granules in normal and isoproterenol treated rat submandibular glands. J. Microsc. 113, 185–203 (1978)

    Google Scholar 

  • Srinivasan, R., Chang, W.W.L.: Effect of neonatal sympathectomy on the postnatal differentiation of the submandibular gland of the rat. Cell Tissue Res. 180, 99–109 (1977)

    Google Scholar 

  • Takahama, M., Barka, T.: Electron microscopic alterations of submaxillary gland produced by isoproterenol. J. Ultrastruct. Res. 17, 452–474 (1967)

    Google Scholar 

  • Tamarin, A.: Submaxillary gland recovery from obstruction. II. Electron microscopic alterations of acinar cells. J. Ultrastruct. Res. 34, 288–302 (1971)

    Google Scholar 

  • Venable, J.H., Coggeshall, R.: A simplified lead citrate strain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965)

    Google Scholar 

  • Watson, M.L.: Staining of tissue sections for electron microscopy with heavy medals. J. Biophys. Biochem. Cyto. 4, 475–478 (1958)

    Google Scholar 

  • Yamashina, S., Barka, T.: Localization of peroxidase activity in the developing submandibular gland of normal and isoproterenol treated rats. J. Histochem. Cytochem. 20, 855–872 (1972)

    Google Scholar 

  • Yamashina, S., Mizuhira, V.: Postnatal development of acinar cells in rat submandibular gland as revealed by electron microscopic staining for carbohydrates. Am. J. Anat. 146, 211–236 (1976)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Alabama in Birmingham, University Station, 35294, Birmingham, Alabama, USA

    J. H. Sheetz & L. Menaker

  2. Department of Oral Biology and Institute of Dental Research, School of Dentistry, University of Alabama in Birmingham, Birmingham, Alabama, USA

    J. H. Sheetz & L. Menaker

Authors
  1. J. H. Sheetz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Menaker
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported in part by NIDR grant DE-02670.

The authors wish to thank Dr. C.A. Schneyer for her comments in the preparation of this paper

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sheetz, J.H., Menaker, L. Morphological and functional study of the effect of isoproterenol on salivary gland cells. Cell Tissue Res. 203, 321–329 (1979). https://doi.org/10.1007/BF00237246

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00237246

Key words

Search

Navigation