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Thiamine deficiency in the cat leads to severe learning deficits and to widespread neuroanatomical damage

Experimental Brain Research volume 48, pages 199–208 (1982)Cite this article

Summary

Behavioral and neuroanatomical consequences of a thiamine-deficient diet, combined with the application of a thiamine-antagonist (pyrithiamine) were investigated in the cat. Eight cats (the experimental group) were subjected to a vitamine-B1-poor diet until they developed neurological symptoms (epileptic attacks, ataxia, gait disturbances), while 24 cats were fed normally and served as control group. Immediately following the appearance of neurological signs, a high dose of thiamine was given to the cats of the experimental group; they were then allowed to recover for ten days. Thereafter the performance in learning an alternation task in a T-maze was tested and compared with that of the control group. Behaviorally, the cats of the experimental group manifested drastically retarded acquisition rates in the learning task compared to the cats of the control group. Neuroanatomically, damage was found in the brains of each of the cats in the experimental group; this damage consisted mainly of enlarged ventricles, hemorrhages, neuronal loss and gliosis. Those regions most consistently affected were the periaqueductal gray, the inferior colliculi and the mamillary nuclei. The thalamic mediodorsal nucleus was affected to a minor degree in three cats only. Four cats manifested damage in the hippocampal formation. No damage was found in the cerebellum. Most of the damaged neuroanatomical loci resemble those found in patients with a Wernicke-Korsakoff syndrome.

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References

  • Adams RD (1969) The anatomy of memory mechanisms in the human brain. In: Talland GA, Waugh NC (eds) The pathology of memory. Academic Press, New York, pp 91–106

    Google Scholar 

  • Bai P, Bennion M, Gubler CJ (1971) Biochemical factors involved in the anorexia of thiamine deficiency in rats. J Nutr 101: 731–738

    Google Scholar 

  • Behse F, Buchthal F (1977) Alcoholic neuropathy: Clinical, electrophysiological, and biopsy findings. Ann Neurol 2: 95–110

    Google Scholar 

  • Ben-Ari Y, Tremblay E, Riche D, Ghilini G, Naquet R (1981) Electrographic, clinical and pathological alterations following systemic administration of kainic acid, bicuculline or pentetrazole: Metabolic mapping using the deoxyglucose method with special reference to the pathology of epilepsy. Neuroscience 6: 1361–1391

    Article  CAS  PubMed  Google Scholar 

  • Bender L, Schilder P (1933) Encephalopathia alcoholica (Polioencephalitis haemorrhagica superior of Wernicke). Arch Neurol Psychiat 29: 990–1053

    Google Scholar 

  • Bernhardt KS, Herbert R (1937) A further study of vitamin B deficiency and learning with rats. J Comp Physiol Psychol 24: 263–267

    Google Scholar 

  • Blank NK, Vick NA, Schulman S (1975) Wernicke's encephalopathy. An experimental study in the rhesus monkey. Acta Neuropathol (Berl) 31: 137–150

    Google Scholar 

  • Brožek J, Vase G (1961) Experimental investigations on the effects of dietary deficiencies on animal and human behavior. Vitam Horm 19: 43–94

    Google Scholar 

  • Campbell ACP, Biggart JH (1939) Wernicke's encephalopathy (Polioencephalitis haemorrhagica superior): Its alcoholic and non-alcoholic incidence. J Pathol Bacteriol 48: 245–262

    Google Scholar 

  • Cerecedo LR (1955) Thiamine antagonists. Am J Clin Nutr 3: 273–281

    Google Scholar 

  • Collins RC, Kirkpatrick JB, McDougal DB, Jr (1970) Some regional pathologic and metabolic consequences in mouse brain pyrithiamine-induced thiamine deficiency. J Neuropathol Exp Neurol 29: 57–69

    Google Scholar 

  • Cruickshank EK (1976) Effects of malnutrition on the central nervous system and the nerves. In: Vinken PJ, Bruyn GW (eds) Handbook of clinical neurology, vol 28, part II: Metabolic and deficiency diseases of the nervous system. Elsevier/North-Holland, Amsterdam, pp 1–41

    Google Scholar 

  • Davison C, Stone L (1937) Lesions of the nervous system of the rat in vitamin B deficiency. Arch Pathol 23: 207–223

    Google Scholar 

  • DeOlmos JS, Ingram WR (1971) An improved cupric-silver method for impregnation of axonal and terminal degeneration. Brain Res 33: 523–529

    Google Scholar 

  • Drachman DA, Arbit J (1966) Memory and the hippocampal complex. Arch Neurol 15: 52–61

    Google Scholar 

  • Dreyfus PM, Victor M (1961) Effects of thiamine deficiency on the central nervous system. Am J Clin Nutr 9: 414–425

    Google Scholar 

  • Fritz MF (1932) Maze performance of the white rat in relation to unfavorable salt mixture and vitamin B deficiency. J Comp Physiol Psychol 13: 365–389

    Google Scholar 

  • Grunnet ML (1969) Changing incidence, distribution and histopathology of Wernicke's polioencephalopathy. Neurology 19: 1135–1139

    Google Scholar 

  • Hakim AM, Pappius HM (1981) The effect of thiamine deficiency on local cerebral glucose utilization. Ann Neurol 9: 334–339

    Google Scholar 

  • Irle E, Markowitsch HJ (1982) Single and combined lesions of the cat's thalamic mediodorsal nucleus and the mamillary bodies lead to severe deficits in the acquisition of an alternation task. Behav Brain Res (in press)

  • Joliffe N, Wortis H, Fein HD (1941) The Wernicke syndrome. Arch Neurol Psychiat 46: 569–597

    Google Scholar 

  • Jubb KV, Saunders LZ, Coates HV (1956) Thiamine deficiency encephalopathy in cats. J Comp Pathol 66: 217–227

    Google Scholar 

  • Langohr HD, Petruch F, Schroth G (1981) Vitamin B1, B2 and B6 deficiency in neurological disorders. J Neurol 225: 95–108

    Google Scholar 

  • Lee K, Dunwiddie T, Deitrich R, Lynch G, Hoffer B (1981) Chronic ethanol consumption and hippocampal neuron dendritic spines: A morphometric and physiological analysis. Exp Neurol 71: 541–549

    Google Scholar 

  • Mair WGP, Warrington EK, Weiskrantz L (1979) Memory disorder in Korsakoff psychosis. A neuropathological and neuropsychological investigation of two cases. Brain 102: 749–783

    Google Scholar 

  • Mancall EL, McEntee WJ (1965) Alterations of the cerebellar cortex in nutritional encephalopathy. Neurology 15: 303–313

    Google Scholar 

  • Markowitsch HJ (1982) Retention performance of a learned delayed-alternation task after chemical lesions of the cat's mediodorsal nucleus. Behav Brain Res 4: 263–277

    Google Scholar 

  • Markowitsch HJ, Pritzel M, Kessler J, Guldin W, Freeman RB, Jr (1980) Delayed-alternation performance after selective lesions within the prefrontal cortex of the cat. Behav Brain Res 1: 67–91

    Google Scholar 

  • Maurer S (1935) III. The effect of partial depletion of vitamin B (B1) upon performance in rats. J Comp Physiol Psychol 20: 309–317

    Google Scholar 

  • Maurer S, Tsai LS (1931) The effect of partial depletion of vitamin B complex upon learning ability in rats. J Nutr 4: 507–516

    Google Scholar 

  • Meyer JG, Neundörfer B, Rethel R, Walker G, Bayerl J (1981) Über die Beziehung zwischen alkoholischer Polyneuropathie und Vitamin b1, B12 und Folsäure. Nervenarzt 52: 329–332

    Google Scholar 

  • O'Neill PH (1949) The effect on subsequent maze learning ability of graded amounts of vitamin B1 in the diet of very young rats. J Genet Psychol 74: 85–95

    Google Scholar 

  • Phillips GB, Victor M, Adams RD, Davidson CS (1952) A study of the nutritional defect in Wernicke's syndrome. The effect of purified diet, thiamine, and other vitamins on the clinical manifestations. J Clin Invest 31: 859–871

    Google Scholar 

  • Poe CF, Poe E, Muenzinger KF (1937) The effect of vitamin deficiency upon the acquisition and retention of the maze habit in the white rat. J Comp Physiol Psychol 23: 67–76

    Google Scholar 

  • Poe E, Poe CF, Muenzinger KF (1936) The effect of vitamin, deficiency upon the acquisition and retention of the maze habit in the white rat. I. The vitamin B-complex. J Comp Physiol Psychol 22: 69–77

    Google Scholar 

  • Poe E, Poe CF, Muenzinger KF (1939) The effect of vitamin deficiency upon the acquisition and retention of the maze habit in the white rat. IV. Vitamins B-complex, B1, and B2 (G). J Comp Physiol Psychol 27: 211–214

    Google Scholar 

  • Ponsford JL, Donnan GA (1980) Transient global amnesia — a hippocampal phenomenon? J Neurol Neurosurg Psychiat 43: 285–287

    Google Scholar 

  • Riggs HE, Boles RS (1944) Wernicke's disease. A clinical and pathological study of 42 cases. Q J Stud Alcohol 5: 361–370

    Google Scholar 

  • Riley JN, Walker DW (1978) Morphological alterations in hippocampus after long-term alcohol consumption in mice. Science 201: 646–648

    Google Scholar 

  • Rindi G, Perri V, DeCaro L (1961) The uptake of pyrithiamine by cerebral tissue. Experientia 17: 546–547

    Google Scholar 

  • Rinehart JF, Friedman M, Greenberg LD (1949) Effect of experimental thiamine deficiency on the nervous system of the rhesus monkey. Arch Pathol 48: 129–139

    Google Scholar 

  • Still CN (1976) Nicotinic acid and nicotinamide deficiency: Pellagra and related disorders of the nervous system. In: Vinken PJ, Bruyn GW (eds) Handbook of clinical neurology, vol 28, part II: Metabolic and deficiency diseases of the nervous system. Elsevier/North-Holland, Amsterdam, pp 59–104

    Google Scholar 

  • Troncosco JC, Johnston MV, Hess KM, Price DL (1981) Model of Wernicke's encephalopathy. Arch Neurol 38: 350–354

    Google Scholar 

  • Van Buren JM, Borke RC (1972) The mesial temporal substratum of memory. Brain 95: 599–632

    Google Scholar 

  • Victor M (1976) The Wernicke-Korsakoff syndrome. In: Vinken PJ, Bruyn GW (eds) Handbook of clinical neurology, vol 28, part II: Metabolic and deficiency diseases of the nervous system. Elsevier/North-Holland, Amsterdam, pp 243–270

    Google Scholar 

  • Victor M, Adams RD (1961) On the etiology of the alcoholic neurological diseases. Am J Clin Nutr 9: 379–397

    Google Scholar 

  • Victor M, Adams RD, Collins GH (1971) The Wernicke-Korsakoff syndrome. Blackwell, Oxford

    Google Scholar 

  • Victor M, Angevine J, Mancall E, Fisher CM (1961) Memory loss with lesions of hippocampal formation. Arch Neurol 5: 244–263

    Google Scholar 

  • Victor M, Yakovlev PI (1955) Korsakoff's psychic disorder in conjunction with peripheral neuritis. A translation of Korsakoff's original article with brief comments on the author and his contribution to clinical medicine. Neurology 5: 394–406

    Google Scholar 

  • Vorhees CV (1979) Avoidance deficits in rats after recovery from mild to moderate thiamin deficiency. Behav Neurol Biol 25: 398–405

    Google Scholar 

  • Walker DW, Barnes DE, Riley JN, Hunter BE, Zornetzer SF (1980a) Neurotoxicity of chronic alcohol consumption: An animal model. In: Sandler M (ed) Psychopharmacology of alcohol. Raven Press, New York, pp 17–31

    Google Scholar 

  • Walker DW, Barnes DE, Zornetzer SF, Hunter BE, Kubanis P (1980b) Neuronal loss in hippocampus induced by prolonged ethanol ingestion in rats. Science 209: 711–713

    Google Scholar 

  • Walker DW, Hunter BE (1978) Short-term memory impairment following chronic alcohol consumption in rats. Neuropsychologia 16: 545–553

    Google Scholar 

  • Watanabe I, Kanabe S (1978) Early edamatous lesion of pyrithiamine induced acute thiamine deficient encephalopathy in the mouse. J Neuropathol Exp Neurol 37: 401–413

    Google Scholar 

  • Watanabe I, Tomita T, Hung K-S, Iwasaki Y (1981) Edamatous necrosis in thiamine-deficient encephalopathy of the mouse. J Neuropathol Exp Neurol 40: 454–471

    Google Scholar 

  • Woolley DW, White AGC (1943) Production of thiamine deficiency disease by the feeding of a pyridine analogue of thiamine. J Biol Chem 149: 285–289

    Google Scholar 

  • Zimmerman HM, Burack E (1932) Lesions of the nervous system resulting from deficiency of the vitamin B complex. Arch Pathol 13: 207–232

    Google Scholar 

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Authors and Affiliations

  1. Dept. of Psychology, University of Konstanz, P.O. Box 5560, D-7750, Konstanz, Germany

    E. Irle & H. J. Markowitsch

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  1. E. Irle
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  2. H. J. Markowitsch
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Irle, E., Markowitsch, H.J. Thiamine deficiency in the cat leads to severe learning deficits and to widespread neuroanatomical damage. Exp Brain Res 48, 199–208 (1982). https://doi.org/10.1007/BF00237215

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  • DOI: https://doi.org/10.1007/BF00237215

Key words

  • Thiamine
  • Vitamin B1
  • Cat
  • Amnesia
  • Brain lesions