Abstract
In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase II and ß-glucuronidase activities and the presence in their genome of integrated bacterial DNA.
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Abbreviations
- 6-BAP:
-
6-benzyl-aminopurine
- CaMV:
-
cauliflower mosaic virus
- GUS:
-
ß-glucuronidase
- IAA:
-
indole-3-acetic acid
- Kn:
-
kanamycin
- MU:
-
methyl umbelliferone
- NAA:
-
naphthaleneacetic acid
- NPTII:
-
neomycin phosphotransferase II
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Communicated by M. R. Davey
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Fontana, G.S., Santini, L., Caretto, S. et al. Genetic transformation in the grain legume Cicer arietinum L. (chickpea). Plant Cell Reports 12, 194–198 (1993). https://doi.org/10.1007/BF00237052
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DOI: https://doi.org/10.1007/BF00237052