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Leucine-enkephalin-like immunoreactive fibers in the medial preoptic area of the rat: their distribution and origin

Experimental Brain Research volume 60, pages 535–541 (1985)Cite this article

Summary

We studied the distribution of fibers with leucine-enkephalin — like immunoreactivity (L-ENKI) in the medial preoptic area (MPO) of the rat, and the origins of such fibers, using indirect immunofluorescence and a combination of a retrograde tracer with immunocytochemistry that we have developed. These fibers were very dense throughout the rostro-caudal part of the MPO. The distribution was uneven with the highest density in the lateral part. Destruction of the arcuate nucleus, which contains a group of L-ENKI neurons, resulted in the marked reduction of these fibers in the ipsilateral MPO, suggesting that most of these fibers originate in this nucleus. This was also suggested by the fact that injection of biotin-wheat germ agglutinin into the MPO labelled many neurons in the arcuate nucleus ipsilaterally. Simultaneous staining with antiserum showed that some of these neurons are L-ENKI.

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References

  • Bai FL, Yamano M, Shiotani Y, Emson PC, Powell JF, Tohyama M (1985) An arcuate-paraventricular and -dorsomedial hypothalamic neuropeptide Y-containing system which lacks noradrenaline in the rat. Brain Res 331: 172–175

    Google Scholar 

  • Card JP, Brecha N, Moore RY (1983) Immunohistochemical localization of avian pancreatic polypeptide-like immunoreactivity in the rat hypothalamus. J Comp Neurol 217: 123–126

    Google Scholar 

  • Coons AH (1958) Fluorescent antibody method. In: Dabielli JF (ed) General cytochemical methods. Academic Press, New York, pp 399–422

    Google Scholar 

  • Day TA, Willoighby JO, Geffen LB (1979) Thermoregulatory effects of preoptic area injections of restrained and unrestrained rat. Brain Res 174: 175–179

    Google Scholar 

  • Geller EB, Hawk C, Keinath SH, Tallarida RJ, Alder MW (1983) Subclasses of opioids based on body temperature change in rats: acute subcutaneous administration. J Pharmacol Exp Ther 225: 391–398

    Google Scholar 

  • Guillemin R, Ling NM, Burgus R (1976) Endorphins, peptides, d'origine hypothalamique et neurohypophydrsire á activité morphonométique. Isolement et structure moleculaire de l' -endorphine. C R Acad Sci 282: 783–785

    Google Scholar 

  • Grimm CT, Bridges RS (1983) Opiate regulation of maternal behavior in the rat. Pharmacol Biochem Behav 19: 609–616

    Google Scholar 

  • Hökfelt T, Skargerberg G, Skillboll L, Björklund A (1983) Combination of retrograde tracing and neurotransmitter histochemistry. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy, Vol 1. Methods in chemical neuroanatomy. Elsevier, Amsterdam, pp 228–285

    Google Scholar 

  • Jacobson CD, Terkel J, Gorski RA, Sawyer CH (1980) Effects of small medial preoptic area lesions on maternal behavior: retrieving and nest building in the rat. Brain Res 194: 471–478

    Google Scholar 

  • Kandasamy SB, Williams BA (1983) Peptide and non-peptide opioid hyperthermia in rabbit. Brain Res 265: 63–71

    Google Scholar 

  • Kawai Y, Inagaki S, Shiosaka S, Shibasaki T, Ling N, Tohyama M, Shiotani Y (1984) The distribution and projection of γ-melanocyte stimulating hormone in the rat brain: an immunochemical analysis. Brain Res 297: 21–32

    Google Scholar 

  • Khachaturian H, Lewis M, Watson S (1983) Enkephalin system in diencephalon and brainstem of the rat. J Comp Neurol 220: 310–320

    Google Scholar 

  • Lind RW, Swanson LW (1984) Evidence for corticotropin releasing factor and leu-enkephalin in the neural projection from the lateral parabrachial nucleus to the median preoptic nucleus: a retrograde transport, immunohistochemical double labeling study in the rat. Brain Res 321: 217–224

    Google Scholar 

  • Mesulam M-M (1978) Tetramethylbenzidine for horseradish peroxidase neurohistochernistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26: 106–117

    CAS  PubMed  Google Scholar 

  • Micevych PE, Elde RP (1982) Neurons containing α-melanocyte stimulating hormone and β-endorphin immunoreactivity in the cat hypothalamus. Peptides 3: 655–662

    Google Scholar 

  • Morrell JI, Pfaff DW (1982) Characterization of estrogen-containing hypothalamic neurons by their axonal projections. Science 217: 1237–1276

    Google Scholar 

  • Nakayama T, Eisenman JS, Hardy JD (1961) Single unit activity of anterior hypothalamus during local heating. Science 134: 560–561

    Google Scholar 

  • Olson GA, Olson RD, Kastin AJ (1984) Endogenous opiate: 1983. Peptide 5: 975–992

    Google Scholar 

  • Osamura RY, Komatsu N, Watanabe K, Nakai Y, Tanaka I, Imura H (1982) Immunohistochemical and immunocytochemical localization of γ-melanocyte stimulating hormone (γ-MSH)-like immunoreactive reactivity in human and rat hypothalamus. Peptides 3: 781–787

    Google Scholar 

  • Pfaff D, Keiner M (1974) Atlas of estradiol-concentrating cells in the central nervous system of the female rat. J Comp Neurol 151: 121–158

    Google Scholar 

  • Sakanaka M, Senba E, Shiosaka S, Takatsuki K, Inagaki S, Takagi H, Kawai Y, Hara Y, Tohyama M (1982) Evidence for the existence of an enkephalin-containing pathway from the area just ventrolateral to the anterior hypothalamic nucleus to the lateral septal area of the rat. Brain Res 239: 240–244

    Google Scholar 

  • Satinoff E, Valentino D, Teitelbaum P (1976) Thermoregulatory cold-defense deficits in rats with preoptic/anterior hypothalamic lesions. Brain Res Bull 1: 553–565

    Google Scholar 

  • Senba E, Shiosaka S, Hara Y, Inagaki S, Kawai Y, Takatsuki K, Sakanaka M, Iida H, Takagi H, Minagawa H, Tohyama M (1982) Ontogeny of the leucine-enkephalin neuron system of the rat: immunohistochemical analysis. I. Lower brainstem. J Comp Neurol 205: 341–359

    Google Scholar 

  • Shiosaka S, Shibasaki T, Tohyama M (1984) Bilateral α-melanocyte stimulating hormonergic fiber system from zona incerta to cerebral cortex: combined retrograde axonal transport and immunohistochemical study. Brain Res 309: 350–353

    Google Scholar 

  • Shiosaka S, Tohyama M (1985) A new sensitive combination method of immunocytochemistry and biothinized retrograde tracer. J Neurosci Res (in press)

  • Simely RB, Swansson LW, Gorski RA (1984) Demonstration of a sexual dimorphism in the distribution of serotonin immunoreactive fibers in the medial preoptic nucleus of the rat. J Comp Neurol 225: 151–166

    Google Scholar 

  • Squires RD, Jacobson FF (1968) Chronic deficits of temperature regulation produced in cats by preoptic lesions. Am J Physiol 214: 549–560

    Google Scholar 

  • Steinbusch HWM (1981) Distribution of serotonin-immunoreactivity in the central nervous system of the rat. Cell bodies and terminals. Neuroscience 6: 557–618

    Google Scholar 

  • Stumpf WE, Sar M (1977) Steroid hormone target cells in the periventricular brain relationship to peptide producing cells. Fed Proc 36: 1973–1977

    Google Scholar 

  • Takatsuki K, Sakanaka M, Takagi H, Tohyama M, Shiotani Y (1983) Experimental immunohistochemical studies on the distribution an dorigins of substance P in the medial preoptic area of the rat. Exp Brain Res 53: 183–192

    Google Scholar 

  • Tepperman FS, Hirst M, Gowdey CW (1981) Hypothalamic injection of morphine: Feeding and temperature responses. Life Sci 28: 2459–2467

    Google Scholar 

  • Titus JA, Hausgland R, Sharrow SO, Segal DM (1982) Texas red, a hydrophilic, red-emitting fluorephore for use with fluorescin in dual parameter flow microfluorometric and fluorescence microscopic studies. J Immunol Meth 50: 193–204

    Google Scholar 

  • Umegaki K, Shiosaka S, Kawai Y, Shinoda K, Yagura A, Shibasaki T, Ling L, Tohyama M (1983) The distribution of α- melanocyte stimulating hormone (α-MSH) in the central nervous system of rat. An immunohistochemical study. I. Forebrain and upper brain stem. Cell Mole Biol 29: 377–386

    Google Scholar 

  • Watson SJ, Akil H (1979) The presence of two MSH positive cell groups in rat hypothalamus. Eur J Pharmacol 48: 101–103

    Google Scholar 

  • Watson SJ, Richard III CW, Barchas JD (1978) Adrenocorticotropin in rat brain: immunocytochemical localization in cells and axons. Science 200: 1180–1182

    Google Scholar 

  • Zamboni L, De Martino C (1967) Buffered picric acid formaldehyde: a new rapid fixative for electron microscopy. J Cell Biol 35: 148A

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Affiliations

  1. Department of Neuroanatomy, Institute of Higher Nervous Activity, Osaka University Medical School, 4-3-56 Nakanoshima, Kitaku, 530, Osaka, Japan

    M. Yamano, M. Tohyama, S. Shiosaka & Y. Shiotani

  2. Third Department of Internal Medicine, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minamiku, 734, Hiroshima, Japan

    S. Inagaki & S. Kito

Authors
  1. M. Yamano
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  2. M. Tohyama
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  3. S. Shiosaka
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  4. Y. Shiotani
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  5. S. Inagaki
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  6. S. Kito
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Yamano, M., Tohyama, M., Shiosaka, S. et al. Leucine-enkephalin-like immunoreactive fibers in the medial preoptic area of the rat: their distribution and origin. Exp Brain Res 60, 535–541 (1985). https://doi.org/10.1007/BF00236939

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  • DOI: https://doi.org/10.1007/BF00236939

Key words

  • Enkephalin
  • Projection
  • Medial preoptic area
  • Arcuate nucleus
  • Immunocytochemistry
  • Double-staining method