Summary
We measured changes in the protein: creatinine and trypsin inhibitor: creatinine ratios in the urine of six male marathon runners. Samples of urine were collected before the run, immediately after the run and in 6-h collections for 2 days. We found the greatest increase in the protein: creatinine ratio (2.6 times greater) in urine collected immediately after the run and the greatest increase in the trypsin inhibitor: creatinine ratio in urine samples collected 6-12 and 12-18 h after the run (2 and 3 times greater, respectively). This suggests the existence of different mechanisms for these two physiological processes. The later increase in the trypsin inhibitor: creatinine ratio was perhaps, due to a state of short-lived inflamation and shock after severe physical effort.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bakonska-Pacon E, Borkowski J, Sobiech KA (1990) Changes of acute phase parameters in serum after the Marathon run (in Polish, abstract in English). Monograph AWF, Poznan (accepted for publication)
Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Chase T, Shaw E (1967) p-Nitrophenyl-p-Guanidinobenzoate HCI. A new active site titrant for trypsin. Biochim Biophys Res Commun 29:508–514
Faarvang HJ (1965) The urinary trypsin inhibitor in man (Mingin). Scand J Clin Lab Invest 17 [Suppl 83]:1–78
Fritz H, Trautschold T, Werle E (1970) Proteinase inhibitors. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, Weinheim, pp 1066–1071
Ginsberg JM, Chang BS, Matarese PA, Garella S (1983) Use of single voided urine samples to estimate quantitative proteinuria. New Engl J Med 309:1543–1545
Hochstrasser K, Bretzel G, Feuth H, Hilla W, Lempart K (1976) The inter-α-trypsin inhibitor as a precursor of the acid stable trypsin inhibitor in human serum and urine. Hoppe-Seyler's Z Physiol Chem 357:153–162
Lemann J Jr, Doumas BT (1987) Proteinuria in health and disease assessed by measuring the urinary protein/creatinine ratio. Clin Chem 33:297–299
Nowak S (1968) The urinary trypsin inhibitors in some diseases. Enzymol Biol Clin 9:41–52
Onishi H, Yano T, Kato K, Inaba A, Kosuzume H (1985) Effects of human urinary trypsin inhibitor against operative stress. Folia Pharmacol Jpn (in Japanese, abstract in English) 85:16
Poortmans JR (1984) Exercise and renal function. Sports Medicine 1:125–153
Poortmans JP, Wolfs JC, Rampaer L, Niset G, Seillier M (1983) Renal protein excretion after exercise in man. Med Sci Sports Exerc 15:157
Poortmans JR, Brauman H, Staroukine M, Verniory A, Decaestecker Ch, Leclercq R (1988) Indirect evidence of glomerular/ tubular mixed-type postexercise proteinuria in healthy humans. Am J Physiol 23:F277–283
Pratt CW, Swaim MW, Pizzo SV (1989) Inflammatory cells degrade inter-α-inhibitor to liberate urinary proteinase inhibitors. J Leukocyte Biol 45:1–9
Shaw AB, Risdon P, Levis-Jackson JD (1983) Protein/creatinine index and Albustix in assessment of proteinuria. Br Med J 287:929–932
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Borkowski, J., A. Sobiech, K. Protein: creatinine and trypsin inhibitor: creatinine ratios in the urine of marathon runners. Eur J Appl Physiol 61, 124–127 (1990). https://doi.org/10.1007/BF00236705
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00236705