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Somatic embryogenesis from leaf protoplasts of Rauvolfia vomitoria shoot cultures

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Abstract

Rauvolfia vomitoria mesophyll protoplasts have been isolated from axenic shoot cultures and cultured (105-106 protoplasts per ml) in Murashige and Tucker liquid medium containing growth regulators. Within 6–8 weeks, a mixed population of calli and proembryos were obtained and transferred on solid media. Calli produced shoots; however, rooting did not occur. Somatic embryos achieved different patterns of development. In particular, whole plantlets have been obtained either directly through germination of primary embryos or via embryogenic calli.

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Abbreviations

B5:

Gamborg et al. (1968) medium

BA:

N6 (benzyl) adenine

2,4-D:

2,4 dichlorophenoxyacetic acid

MT:

Murashige and Tucker (1969) medium

NAA:

naphthalene acetic acid

Z:

zeatin

K:

kinetin

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire et Biochimie Végétale, Faculté de Pharmacie, 2bis Boulevard Tonnellé, 37042, Tours cédex, France

    Jocelyne Trémouillaux-Guiller & Jean-Claude Chénieux

Authors
  1. Jocelyne Trémouillaux-Guiller
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  2. Jean-Claude Chénieux
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Communicated by I. Potrykus

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Trémouillaux-Guiller, J., Chénieux, JC. Somatic embryogenesis from leaf protoplasts of Rauvolfia vomitoria shoot cultures. Plant Cell Reports 10, 102–105 (1991). https://doi.org/10.1007/BF00236467

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  • DOI: https://doi.org/10.1007/BF00236467

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