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Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea

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Summary

Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea. Comparative HPLC fingerprint analysis of the alkamides from natural plant source, control tissues, and transformed callus and roots indicated that transformed callus and hairy roots might be a promising source for continuous and standardized production of the dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide and related amides.

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Abbreviations

HPLC:

high-pressure liquid chromatography

MS:

Murashige and Skoog culture medium

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Authors and Affiliations

  1. Laboratory of Pharmacognosy and Phytochemistry, State University of Ghent, Harelbekestraat 72, B-9000, Gent, Belgium

    M. Trypsteen & R. Van Severen

  2. Laboratory of Genetics, State University of Ghent, Ledeganckstraat 35, B-9000, Gent, Belgium

    M. Van Lijsebettens & M. Van Montagu

Authors
  1. M. Trypsteen
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  2. M. Van Lijsebettens
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  3. R. Van Severen
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  4. M. Van Montagu
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Communicated by W. Bary

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Trypsteen, M., Van Lijsebettens, M., Van Severen, R. et al. Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea . Plant Cell Reports 10, 85–89 (1991). https://doi.org/10.1007/BF00236463

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  • DOI: https://doi.org/10.1007/BF00236463

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