Abstract
The percentage of green plants in anther culture is known to be controlled by the genetics of anther donor materials. The objective of this study was to determine whether components in the culture media also would have a significant influence on the percentage of green plants from wheat anther culture. Anthers of a spring wheat cultivar, “Pavon 76”, were cultured on potato 4 (P4) induction media with various modifications. Addition of 200 g/l ficoll to the liquid P4 medium significantly increased the percentage of green plants even though the final yield of green plants per 100 anthers was lower than the liquid medium. A higher concentration of maltose (135 g/l) produced significantly higher percentage of green plants than the medium containing 90 g/l maltose or sucrose. These results demonstrate culture medium effects on albinism, indicating that the percentage of green plants in wheat anther culture can be increased by optimizing medium osmotic potential.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agache S, Bachelier B, de Buyser J, Henry Y, Snape J (1989) Theor Appl Genet 77: 7–11
Bjornstad A, Opsahl-Ferstad HG, Aasmo M (1989) Plant Cell Tissue Organ Culture 17: 27–37
Bullock WP, Baenziger PS, Schaeffer GW, Bottino PJ (1982) Theor Appl Genet 62: 155–159
Chen Y (1986) In: Hu H, Yang H (ed) Haploids of higher plants in vitro. China Academic Publishers, Beijing, pp 3–25
Ekiz H, Konzak CF (1990) Crop Science, in press
Finnic SJ, Powell W, Dyer AF (1989) Plant Breeding 103: 110–118
Henry Y, de Buyser J (1985) Plant Cell Reports 4: 307–310
Huang B (1987) Plant Cell Tissue Organ Culture 9: 45–48
Jones AM, Petolino JF (1988) Plant Cell Tissue Organ Culture 12: 253–261
Kao KN (1981) Z. Pflanzenzuchtg 103: 437–443
Kasha KJ, Ziauddin A, Cho UH (1989) XIX Stadler Genetics Symp. Missouri, pp.213–236
Knudsen S, Due IK, Andersen SB (1989) Plant Breeding 103: 241–246
Last DI, Brettell RIS (1990) Plant Cell Reports 9: 14–16
Olsen FL (1987) Carlsberg Res Commun 52: 393–404
Orshinsky BR, McGregor LJ, Johnson GIE, Hucl P, Kartha KK (1990) Plant Cell Reports 9: 365–369
Ouyang JW (1986) In: Hu H, Yang HY (ed) Haploids of higher plants in vitro, China Academic Publishers, Beijing, pp 26–41
Ouyang JW, Zhou SM, Jia SE (1983) Theor Appl Genet 66: 101–109
Roberts-Oehlschlager SL, Dunwell JM (1990) Plant Cell Tissue Organ Culture 20: 235–240
SAS (1988) SAS/STAT user's guide, release 6.03 edition. SAS Institute Inc., Cary, NC, USA
Simmonds J (1989) Plant Science 65: 225–231
Sorvari S, Schieder O (1987) Plant Breeding 99: 164–171
Szakacs E, Kovacs G, Pauk J, Barnabas B (1988) Plant Cell Reports 7:127–129
Tuvesson IKD, Pedersen S, Andersen SB (1989) Theor Appl Genet 78:879–883
Zhang LJ, Anceau C, Lepoivre P, Seilleur P, Semal J (1987) Bull Rech Agron Gembloux 22: 301–314
Zhou H, Konzak CF (1989) Crop Science 29: 817–821
Zhuang JJ, Xu J (1983) In: Hu H, Vega MR (ed) Cell and tissue culture techniques for cereal crop improvement. Sci. Press, Beijing, PRC, pp 431
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Rights and permissions
About this article
Cite this article
Zhou, H., Zheng, Y. & Konzak, C.F. Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Reports 10, 63–66 (1991). https://doi.org/10.1007/BF00236458
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00236458