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ATP activates a Ca2+-dependent Cl− current in the rat thyroid cell line, FRTL-5

The Journal of Membrane Biology volume 125, pages 243–253 (1992)Cite this article

Summary

The effect of external ATP on both the membrane potential and the transmembrane current of the thyroid cell line FRTL-5 has been investigated in the patch-clamp whole-cell recording configuration. In the resting situation the membrane potential is around −70 mV and the membrane acts like a K+-sensitive electrode. Application of ATP at concentrations higher than 1 μ M elicited an increase in Cl− conductance, responsible for a membrane depolarization which could be blocked by preincubation with the P2-antagonist quinidine. Chelation of intracellular Ca2+ also blocked the ATP induced changes in membrane potential and Cl− current. Intracellular perfusion with inositol trisphosphate (IP3) (50 μ M) also stimulated a Cl− current which mimicked the response induced by ATP. ATP is able to initiate a response in the absence of extracellular Ca2+, but also opens a Ca2+-influx pathway, as demonstrated by a secondary response upon Ca2+ readmission in the external medium, in the continued presence of ATP. ADP and ATP-γS were able to mimic the ATP response, whereas AMP and adenosine were unable to elicit a Cl− current. The P2X receptor agonist α,β-methyleneATP was without effect as was the P2Y receptor agonist 2-methylthio ATP. We conclude that ATP is able to elicit a large IP3-mediated Ca2+-dependent Cl− current and membrane depolarization via a novel P2-type purinergic receptor.

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Affiliations

  1. The MRC Secretory Control Research Group, The Physiological Laboratory, University of Liverpool, L69 3BX, Liverpool, England

    S. C. Martin

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  1. S. C. Martin
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I would like to thank Prof. O.H. Petersen for his constructive advice on the preparation of this manuscript, Dr. D. Corda of the S. Maria Imbaro Institute for her invaluable guidance, Dr. P. Smith for the computer software used for data collection and analysis, and Alan Higgins for efficient technical assistance. This work was supported by an MRC Programme Grant (OHP). SCM is in receipt of a University of Liverpool Postgraduate Student Grant.

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Martin, S.C. ATP activates a Ca2+-dependent Cl− current in the rat thyroid cell line, FRTL-5. J. Membarin Biol. 125, 243–253 (1992). https://doi.org/10.1007/BF00236437

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  • DOI: https://doi.org/10.1007/BF00236437

Key words

  • ATP
  • FRTL-5
  • thyroid gland
  • chloride currents
  • inositol trisphosphate
  • calcium