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Protoplasts from cotyledon and hypocotyl of sunflower (Helianthus annuus L.): shoot regeneration and seed production

Summary

Cotyledon and hypocotyl protoplasts of Helianthus annuus inbred line 47 302 bcd were embedded in alginate and plated on L4 medium (Lenée and Chupeau 1986). After one month, the calli were transferred on MSSH regeneration medium (Murashige and Skoog 1962; Schenk and Hildebrandt 1972) where they regenerated shoots (overall efficiency 10−2%). The shoots were elongated on B5 (Gamborg et al. 1968) medium first without hormones, then supplemented with GA3 and BAP (both 0.05 mg/l). In order to overcome the difficulty to induce rooting by classical methods, the elongated shoots were grafted on a sunflower rootstock. The grafted shoots produced flowers and seeds. Different factors have been shown to have an important influence on the capacity to regenerate shoots: the genotype, the physical culture conditions at the callus regeneration step (e.g. protoplasts embedded in alginate), and the media composition.

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Abbreviations

BAP:

6-benzylaminopurine

GA3:

gibberellic acid

IBA:

indole-3-butanoic acid

IAA:

indole acetic acid

MES:

2-N-morpholinoethane sulfonic acid

NAA:

1-naphthalene acetic acid

2,4D:

2,4 dichlorophenoxyacetic acid

References

  • Aguilar ME, Villalobos VM, Vasquez N (1992) In vitro Cell. Dev. Biol. 28:15–19.

    Google Scholar 

  • Binding H, Nehls R, Kock R, Finger J, Mordhorst G (1981) Z. Planzenphysiol. 101:119–130.

    Google Scholar 

  • Bohorova NE, Atanassof A, Georgieva-Todorova J (1985) Z. Planzenphysiol. 95:37–44.

    Google Scholar 

  • Bomman CH, Vogelman TC (1984) Physiol. Plant. 61:505–512.

    Google Scholar 

  • Böttcher I, Zoglauer K, Göring H (1988) Physiol. Plant. 72:560–564.

    Google Scholar 

  • Burrus M, Chanabé C, Alibert G, Bidney D (1991) Plant Cell Rep. 10:161–166.

    Google Scholar 

  • Chanabé C, Burrus M, Bidney D, Alibert G (1991) Plant Cell Rep. 9:635–638.

    Google Scholar 

  • Chraibi KM, Latche A, Roustan JP, Fallot J (1991) Plant Cell Rep. 10:204–207.

    Google Scholar 

  • Daguin F, Letouze R (1986) Physiol. Plant. 66:94–98.

    Google Scholar 

  • Damm B, Willmitzer L (1988) Mol. Gen. Genet. 213:15–20.

    Google Scholar 

  • Damm B, Schmidt R, Willmitzer L (1989) Mol. Gen. Genet. 217:6–12.

    Google Scholar 

  • Debergh PC, Harbaoui Y, Lemeur R (1981) Physiol. Plant. 53:181–187.

    Google Scholar 

  • Dillen W, Buysens S (1989) Plant Cell Tissue Org. Cult. 19:181–188.

    Google Scholar 

  • Finer JJ (1987) Plant Cell Rep. 6:372–374.

    Google Scholar 

  • Fischer C, Hahne G (1992) Protoplasma, in press.

  • Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158.

    Google Scholar 

  • Greco B, Tanzarella OA, Carrozzo G, Blanco A (1984) Plant Sci. Lett. 36:73–77.

    Google Scholar 

  • Jeannin G, Hahne G (1991) Plant Breeding 107:280–287.

    Google Scholar 

  • Knittel N, Escandon A, Hahne G (1991) Plant Sci. 73:219–226.

    Google Scholar 

  • Krasnyanski S, Polgar Z, Németh G, Menczel L (1992) Plant Cell Rep. 11:7–10.

    Google Scholar 

  • Lenée P, Chupeau Y (1986) Plant Sci. 43:69–75.

    Google Scholar 

  • Leshem B (1983) Ann. Bot. 52:413–415.

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497.

    Google Scholar 

  • Natali L, Cavallini A (1989) Plant Breeding 102:341–343.

    Google Scholar 

  • Orlikowska T (1987) Acta Hort. 212:237–244.

    Google Scholar 

  • Paterson KE (1984) Amer. J. Bot. 71:925–931.

    Google Scholar 

  • Perl A, Aviv D, Galun E (1988) Plant Cell Rep. 7:403–406.

    Google Scholar 

  • Rugini E (1986) Almonds. In: Handbook of Plant Cell Culture, Vol 4 (DA Evans et al.) Macmillan Pub. Co, London, pp 574–611.

    Google Scholar 

  • Schenk RU, Hildebrandt AC (1972) Can. J. Bot. 50:199–204.

    Google Scholar 

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Communicated by H. Lörz

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Fischer, C., Klethi, P. & Hahne, G. Protoplasts from cotyledon and hypocotyl of sunflower (Helianthus annuus L.): shoot regeneration and seed production. Plant Cell Reports 11, 632–636 (1992). https://doi.org/10.1007/BF00236388

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  • DOI: https://doi.org/10.1007/BF00236388

Key words

  • Helianthus annuus
  • Protoplasts
  • Plant regeneration